CHANGEs OF characteristics of erythrogenesis in blood of rabbits INACTIVATED with multivalent vaccine against leptospirosis in CATTLE by intramuscularly injection

V. Ukhovskyi, A. Rozumnyuk

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Institute of Veterinary Medicine NAAS, Donetska st., 30, Kyiv, 03151, Ukraine

The article presents the results on a study of erythrogenesis peripheral blood characteristics in rabbits (quantity of erythrocyte, hemoglobin concentration, medium volume of erythrocyte, medium volume of hemoglobin in one erythrocyte, Hematocrit value) that were immunized with inactivated polyvalent vaccine against leptospirosis in animal «variant bovis».

The test was conducted in rabbits selected on the basis of analogues (9 experimental and 5 control groups). Animals of the experimental group were immunized with an inactivated vaccine in cattle against leptospirosis «Polyvalent vaccine against leptospirosis in animals (variant bovis)», produced by Institute of Veterinary Medicine of NAAS, which includes the cell suspension of the strains Leptospira interrogans of serogroups: Grippotyphosa (VGNKI-1 strain), Icterohaemorrhagiae (strain VGNKI-2), Tarassovi (strain VGNKI-4), Hebdomadis (strain Kabura), Sejroe (strain 493 and Poland Hardjoprajtno), the inactivator (formalin), sorbent and adjuvant (polyethylene glycol) and the water. The vaccine was injected intramuscularly with the dose of 0.75 cm3 per animal. The vaccine was not injected in control group of animals.

Research results indicate a change in the application of indicators erythrogenesis using of the inactivated vaccine, which are manifested in the form of short-term oligochrome erythrocytopenia. Hemoglobin concentration in peripheral blood of rabbits after injection of the vaccine against leptospirosis is reduced by 7%(from 114±2.9 till 105±3.1 gr/l; р<0.05; 7-th day), but the quantity of erythrocytesby 7.7 % (р<0.05),compared to the beginning of the research. This is due to the influence of leptospira endotoxin contained in the vaccine and cause hemolysis of erythrocytes. Confirmation of results serve as the definition of hematocrit values, which on the 7th day of the experiment decreased by 8.1% (p<0.05). Further researches (14 and 21 days) indicated an inverse trend.The above characteristics do not only restored to the original and even exceed their values: hemoglobin concentration 8 (p<0.05) and 18 % (p<0.001), respectively; red blood cells 10 (p<0.05) and 23 % (p<0.001); hematocrit value 5 and 16% (p<0.05). However, the data obtained on the 28th day pointed to a gradual decrease in these parameters, indicating restoration of physiological erythropoethis. Calculate the average volume of red blood cells in peripheral blood of rabbits given the opportunity to confirm the intensification of metabolic processes in animals under the influence of the vaccine, which causes premature aging of red blood cells, which have a smaller volume compared to the «young» erythrocytes. This rate in the experimental group during the 28 days fell by 10% (p<0.001). The average content of hemoglobin in one erythrocyte throughout the research (28 days) did not change significantly and did not differ significantly as between the experimental and control groups of animals, and the values ​​at the beginning of the experiment (1.30±0.0161.42±0.015 fmol, that does not exceed the physiological fluctuations).

As a result of the studies it was found that the injection of polyvalent vaccine against leptospirosis of animals (variant bovis) to rabbits does not cause a negative impact on performance erythropoethis of animals, and these changes are the result of physiological reactions of animals.

Keywords: VACCINE, Leptospira, leptospirosis, serogroup, Etiology, cattle, erythrogenesis, RABBITS, erythrocytes, hemoglobin, hematocrit

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