MORPHOMETRIC CHARACTERISTIC OF RATS PANCREASATPRE-SLAUGHTER STRESSUNDER USING OF BIOLOGICALLYACTIVE SUBSTANCES

S. S. Grabovskyi

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Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj, 50,Pekarska St., Lviv, 79010, Ukraine

We have carried out studies on morphometric parameters of rat pancreas under pre-slaughter stress conditions using biologically active substances of plant and animal origin. The spleen extract, Echinacea and Chinese lemon extracts (70°alcoholsolutions), sprouted grains were added to the diet of ratsof all experimental groups by aerosol method as an antistressors and immunomodulators in pre-slaughter period (five days beforeslaughter). Only 70°alcoholsolutionin the same volumeand the same method was added to the diet of rats of control group five days beforeslaughter.The experiment continuedfor five days in the vivarium, laboratory animals taken from cagein turn, weighed and decapitated under ether anaesthesia.

    Significant changes in the morphological structure of pancreatic tissue under pre-slaughter stress have not manifested. At morphometric investigationof the pancreas islets Langerhans diameter of rats of all experimental groups showed some differences. In rats, which were added to the diet spleen extract, the area occupied by the islets of Langerhans, was the largest (I experimental group).

The rats of control group, which were not fed with immunomodulators and anti-stress compounds, the signs of the local replacement of the exocrine pancreas, especially in areas around blood vessels, adipose tissuebeen observed, which werewithin normal limits.

These results obtained in model experiments on laboratory animals in the vivarium can be used in research on agricultural animals to prevent the effect of a pre-slaughter animal stress on thequality of a meat product.

 

Keywords: RATS,PANCREAS, PRE-SLAUGHTER STRESS, SPLEEN EXTRACT, ECHINACEA AND CHINESE LEMON EXTRACTS,SPROUTED GRAINS

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