EFFECTS OF EXOGENOUS CHOLESTEROL AND AMIDATED CELLULOSE ON FAT AND STEROLS CONCENTRATION IN FAECES

M. Marounek1, Z. Volek1, J. Tůma2,D. Duškova1, M.S. Kalachniuk3,

L.G. Kalachnyuk3, G.I. Kalachnyuk3

marounek.milan@vuzv.cz, lilkalachnyuk@gmail.com

1Institute of Animal Science, Přátelství 815, 104 00 Prague 22, Czech Republic

2Institute of Chemical Technology, 166 28 Prague 6, Czech Republic

3National Universityof Life and Environmental Sciences of Ukraine, Heroiv Oborony str.,15; Kyiv, 03041, Ukraine

The increased conversion of cholesterol to bile acids in the liver compensates for the loss of bile acids in faeces. This leads to lower cholesterol levels in the blood. An alternative to bile acid sequestrants is sorbents of sterols prepared by amidation of polysaccharides.

There was observed the hypocholesterolemic effect of octadecylpectinamide in rats that were kept on diets containing cholesterol in the amount of 10 g/kg. Amidated pectin reduces cholesterol in the blood serum and significantly increases levels of natural sterols and bile acids in faeces. Therefore we investigated the effect of feeding cholesterol and amidated oxidized monocarboxycellulose (MCC), and carboxymethylcellulose (CMC) on the concentration of fat and sterols in the feces of rats. We used 30 femaleWistar rats aged 3 months and weighing ~ 250 g. After leveling period (4 weeks), rats were divided into 6 groups of 5 animals each. The first group was control, diets of groups (2–6) were supplemented with cholesterol at 10 g/kg at expense of palm fat, diets of 3 and 4 groups supplemented 30 and 60 g/kg of amidated MCC, respectively, instead of conventional cellulose (in total 60 g/kg), and 5 and 6 groups — 30 and 60 g/kg of amidated CMC, respectively. The experiment duration was 4 weeks. Faeces were collected during the last 5 days of the experiment.

It is established that the cholesterol and coprostanol concentration in faeces are significantly increased in all rats which were fed diets supplemented with cholesterol, and coprostanol increases significantly with the consumption of amidated cellulose. Amidated MCC and CMC increase significantly the fecal fat output. They decrease significantly concentration of lithocholic, α-muricholic acids and the total bile acids in faeces.

The results show considerable difference between amidated cellulose and amidated pectin, which significantly increases the fecal excretion of cholesterol and to a lesser extent - of bile acids.

Possibilities of the removal of dietary fat from the organism using the amidated cellulose (that provide a hypolipidemic and hypocholesterolemic effects) will be verified again in the near future.

Keywords: CHOLESTEROL, FAT, BILE ACIDS, AMIDATED CELLULOSE, FAECES, RATS

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