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PHYSIOLOGICAL AND BIOCHEMICAL IMPACT NANOPARTICLES OF CHROMIUM AND SELENIUM CITRATE IN THE BODY YOUNG RATS

R.S. Fedoruk1, N. M. Khomyn2, M. M. Khomyn1

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1Institute of Animal Biology NAAS, st. V. Stus, 38, Lviv, 79034, Ukraine;

2Lviv National University of Veterinary Medicine and Biotechnologies named after S.Z.Gzhytskyj, st. Pekarska, 50, Lviv, 79050, Ukraine

The necessity of the study of biological effects of citrate nanoparticles of Cr and Se, as of essential, but not enough studied elements towards physiological and biochemical mechanisms of their action in mammals in general, and in compounds with organic acids in particular.

The study was carried out in a vivarium of Institute of Animal Biology of NAAS on females-sisters with newborn rats, divided into 5 groups. Control (I) group — female with offspring fed standard diet (SD) of granulated fodder and vegetables. Animals of II (experimental) group with the SD obtained chromium citrate nanoparticles in dose of 37.0 mg Cr/kg of b.w./day via drinking water from day 10 of lactation during period of 60 days. Animals of III (experimental) group — SD and citrate nanoparticles chromium in dose of 74.0 mg Cr/kg of b.w./day. Animals of IV (experimental) group — SD and were applied citrate nanoparticles of chromium and selenium in the amount of 37.0 mg Cr and Se/kg of b.w./day. Rats of V (experimental) group SD and were applied citrate nanoparticles of chromium and selenium in the amount of 74.0 mg Cr and Se / kg of b.w./day.

The study of body weight of females and young rats was conducted during preparatory period, as well as at days 40 and 60 of supplementation. At days 40 and 60 young rats-analogues were decapitated for blood collection and determination of retinol, α-tocopherol, calcium, inorganic phosphorus, cholesterol, triacylglycerols, urea and alkaline phosphatases. During the decapitation a body weight of young rats and weight of their internal organs was determined. In the liver and muscle tissues the content of phenols was analized.

It has been established that the addition to rats of Group III of mineral supplements leads to increased activity of alkaline phosphatase and reduction of blood level of cholesterol, increase of their body weight and also weight of liver and pancreas. Integrated supplementation of animals of group IV by fewer amount citrate nanoparticles of Cr and Se enhanced the intensity of detoxification processes, content of phenolic sulphates and phenolic glucuronide in liver and muscles, increased body mass of young rats and weight of their liver. Under the condition of the increase in citrate nanoparticles Cr and Se content in drinking water of animals of group V, the formation of phenolic sulphates and phenolic glucuronide in liver and muscle tissues remained at the level similar to fourth group but the concentration of free phenols increased and increased the weight of liver and stomach compared with these parameters in animals of control group.

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Keywords: NEWBORN RATS, BLOOD, MUSCLE, LIVER, CHROMIUM, SELENIUM, PHYSIOLOGICAL AND BIOCHEMICAL PROCESSES

© 2016 Institute of Animal Biology

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