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Effect of aflatoxin B1 on the antioxidant system of rat erythrocytes and corrective activity of preparation «Vitakorm-multysporyn»

H. L. Antonyak1,2, Ch. M. Golovchak2, I. V. Luchka1, I. A. Lysik3, I. V. Panchuk4

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1Institute of Animal Biology, NAAS, Laboratory of Metabolism,Stusa St., 38, Lviv, 79034, Ukraine,

2Lviv Ivan Franko National University,Lviv, Saksahanskoho St., 1, 79005, Ukraine

3Scientific and Production Complex «Himtehservis» Ilf and Petrov St., 20, Odessa, 65031, Ukraine

4State Humanitary University of Riwne, Ostafowa St., 29a, Riwne, 33000, Ukraine

Antioxidant system protects cells from damage caused by the action of reactive oxygen (ARO). Excessive formation of ARO in erythrocytes can lead to damage to their structure and violation of biochemical processes. Formation ARO increases under the influence of toxic substances. In extremely toxic compounds include aflatoxins, particularly aflatoxin B1 (AFB1).

Investigated the effects of AFB1 on the activity of antioxidant enzymes and the content of TBA-active products in erythrocytes of rats. The study was conducted on white nonlinear male rats, which were divided into 4 groups of 5 animals each. Animals of the first group was used as control. Rats of the second group were injected with AFB1 concentration of 15 mg/kg daily for 14 days orally. The animals of the third group was administered the same amount of toxin along with the preparation «Vitakorm-Multysporyn». The animals of the fourth group were injected only probiotic preparation.Material for research was animal blood. Blood samples were taken on the 7th and 14th days of the experiment.

It was established that the introduction of AFB1 of laboratory animals on the 7th and 14th day of experiment changes the activity of antioxidant enzymes in animal cells. SOD activity decreased by 36 and 41 % on the 7th and 14th day, respectively, compared with control animals. The activity of catalase in erythrocytes of rats in first experimental group decreased by 60 and 55 % on the 7th and 14th day, respectively, compared with the control group. Glutathione peroxidase activity, in contrast, grew under the influence of AFB1. At the 7th day it increased to 29, and on the 14th 44 %, compared with values in the control group. Glutathione reductase activity on the 7th and 14th day decreased by 63 and 72.5%, respectively, compared with controls. The content of TBA-active products in erythrocytes increased by 31 and 22% on the 7th and 14th day, compared with controls.

Also investigated the influence of probiotic preparation «Vitakorm-Multysporyn» on the antioxidant system in rats aflatoxicosis. With a prolongedadministrationof toxinto rats, to some extent, adapted their to his actions, and the use of the drug «Vitakorm-Multysporyn» significantly stimulates this adaptation.

Keywords: aflatoxin B1, MYCOTOXINS, AFLATOXICOSIS, ANTIOXIDANT SYSTEM, ERYTHROCYTES, PROBIOTIC PREPARATIONS


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