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cellular prion localization and activity of Na+K+- AND Ca2+-ATPases In the spleen of different ages rats

M. V. Kushkevych, V. V. Vlizlo

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Institute of Animal Biology of NAAS, V. Stus str. 38; Lviv79034, Ukraine

An immunohistochemical analysis of spleen tissue of different ages rats are performed. Based on its results the localization of cellular prion (PrPC) is established. The localization of PrPC is unchanged against to the age-related changes of spleen tissue, including the investigated protein was found on the surface of red pulp white blood cells along the spleen trabeculars and near the capsule. The cellular prion in lymphoid follicules is not found. The number change of cellular prion, depending on the rats age, are showed by the immunohistochemical and dot-blotting analysis methods. In particular, the PrPC content is the lowest in one-month rats spleen. The prion number increases in six months aged animals, and thirty-months — decreases. According to the cellular prion is located on the cell membrane outer surface, and it is involved in the Ca2+-transport regulation, since quantitative changes in activity of Ca2+-ATP-ases are associated with PrPC. This relationship is established based on the correlation analysis. The activity decrease of these enzymes with rats age is established. Similar depending between the PrPC number and Na+-K+-ATPase activity is established by the method of correlation analysis, since the enzyme contains in its composition a subunit, that as the cellular prion is sialichlikoproteid. The age-related changes in the activity of the studied ions transporters affect on the ions content. In particular, the content of sodium and calcium ions increases, however potassium ions are significantlyunchanged.

Keywords: spleen, cellular prion, Na+-K+- AND Ca2+-ATPase, sodium ions, potassium and calcium

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