THECONTENT OF TOTAL LIPIDS AND THEIR FACTIONS IN TISSUES OF BREASTS AND BELLY OF MELLIFEROUS BEES AFTER FEEDING OF MEAL FROM BOBS
OF NATIVE AND GENETICALLY MODIFIED SOYBEAN
L. І. Romaniv, R. S. Fedoruk
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Institute of Аnimal Вiology NААS, Stusa str. 38; Lviv, 79034, Ukraine
Influence of compounds of soybean of native and genetically modified sorts has been studied at the conditions of feeding in a spring period on the reproductive function of mothers of bee families, and also on the content of lipids and their factions in tissues of breast and abdominal departments of body of bees. The investigation has been conducted in spring and summer period on the 3 groups of bee families of Carpathian breed of melliferous bees, for 3 families in a group. A control group has been maintained at stationary conditions with the envisaged removal of bee-bread storages 1 time a week. Bees of II experimental group got in the additional feeding a meal of native sort of soybean in an amount 200 g/week/familie, and the bees of III experimental group were fed up by the meal of genetically modified sort of soybean in an amount 200g/week/familie. The investigation lasted 35 days with the interval of the additional feeding of 7 days. Families have been formed of 6 frames of young reproductive mothers, and groups have been selected by the method of selection of pairs-analogues. Control and experience groups of families of bees were safe in relation to infections and invasions. At the finishing stage of an experiment the samples of tissues of melliferous bees have been taken. The content of total lipids and correlation of their separate factions in the experimental samples of tissues of melliferous bees have been determined. The determination of content of total lipids has been conducted by the method of lipid extraction by Folch and correlation of content of separate factions of lipids by the method of TLC with the use of plates with Sorbfil PТLC-P-А. Among total lipids in the breath department of body of melliferous bees of control, II and III experimental groups have been discovered: phosphatides — 18,91; 21,61 (р<0,001); 21,09 (р<0,001); mono- and diacylglycerols — 22,03; 14,70 (р<0,001); 15,62(р<0,001); free cholesterol — 18,57; 16,24 (р<0,001); 14,09(р<0,001); free fatty acids — 14,81; 15,44 (р<0,05); 16,64(р<0,01); triacylglycerols — 14,37; 19,43 (р<0,001); 18,66 (р<0,001); cholesterol ethers — 11,06; 12,52(р<0,01); 13,85(р<0,001). In tissues of abdominal department of body of control bees, II and III experimental groups such changes of content of separate factions of lipids have been shown: phosphatides — 15,01; 22,24 (р<0,001); 22,01 (р<0,001); mono- and diacylglycerols — 17,66; 14,30 (р<0,001); 12,49 (р<0,001); free cholesterol — 21,10; 13,67 (р<0,001); 15,44 (р<0,001); free fatty acids — 16,66; 14,36 (р<0,001); 14,05 (р<0,001); triacylglycerols — 12,93; 15,86 (р<0,001); 17,37 (p<0,001); cholesterol ethers — 16,36; 19,57 (р<0,001);18,59 (р<0,01). The content of phosphatides, free fatty acids, triacylglycerols and cholesterol ethers increased in tissues of breast department of body of melliferous bees of II experimental group. The content mono- and diacylglycerols, free cholesterol reliably decreased. The same increase of content of separate factions of lipids has been observed in muscular tissue of breasts of III experimental group, that got in the additional feeding by meal from the bobs of genetically modified sort of soybean. The content of phosphatides, triacylglycerols and cholesterol ethers increased in the abdominal department of body of melliferous bees of II and III experimental groups as compared to control group. These data can suggest the positive influence of separate compounds of soybean meal on metabolism of total lipids and their separate fractions in tissues of organism of melliferous bees and on level of exchange of it structural elements in the process of life and performance.
Keywords: BEE, PHOSPHOLIPIDS, NEFA, TRIACYLGLYCEROLS, ESTERIFIED CHOLESTEROL, SOY, GMO
