Download full text in PDF
Bìol. Tvarin. 2020; 22 (4): 13–17.
Received 27.11.2020 ▪ Accepted 22.12.2020 ▪ Published online 30.12.2020

State of enzymatic and non-enzymatic links of antioxidant protection of the liver of tumor-bearing rats under the action of laser irradiation

O. V. Ketsa, A. V. Onezhko, M. M. Marchenko

This email address is being protected from spambots. You need JavaScript enabled to view it.

Fedkovich Chernovtsy National University,
2 Kotsiubynskoho str., 58012, Chernivtsi, Ukraine

The activity of antioxidant enzymes — superoxidedismutase (SOD), catalase and glutathionetransferase, and also the level of low molecular weight antioxidants — vitamin E and ascorbic acid in the liver subcellular fractions of rat with transplanted of Guerin’s carcinoma it was investigated. It is shown that in the liver of tumor-bearing rats in the logarithmic phase of oncogenesis increases the activity of the components of the enzymatic link of the antioxidant system (AOS) and the content of vitamin E with a simultaneous decrease of ascorbic acid. The AOS depletionis expressed by a decrease of antioxidant enzymes activity and a decrease the level of low molecular weight antioxidants in the stationary phase of oncogenesis in the liver cells of tumor-bearing rats. It was found that laser irradiation of rats in the area of tumor growth reduces its effect on liver AOS, which is manifested by increased activity of SOD, catalase and vitamin E content in the stationary phase of Guerin’s carcinoma growth in the body.

Key words: superoxidedismutase, glutathionetransferase, catalase, vitamin E, ascorbic acid, Guerin’s carcinoma, laser irradiation, liver

  1. Chikara S, Nagaprashantha LD, Singhal J, Horne D, Awasthi S, Singhal SS. Oxidative stress and dietary phytochemicals: role in cancer chemoprevention and treatment. Cancer Lett. 2018; 413: 122–134.
  2. Farmer C, Abt AF. Determination of reduced ascorbic acid in small amounts of blood. Soc. Biol. Med. 1936; 34 (2): 146–150.
  3. Fujimoto T, Ito S, Ito M, Kanazawa H, Yamaguchi S. Induction of different reactive oxygen species in the skin during various laser therapies and their inhibition by fullerene. Lasers Surg. Med. 2012; 44 (8): 685–694.
  4. Gill JG, Piskounova E, Morrison SJ. Cancer, oxidative stress, and metastasis. Cold Spring Harb. Symp. Quant. Biol. 2016; 81: 163–175.
  5. Glorieux C, Calderon PB. Catalase, a remarkable enzyme: targeting the oldest antioxidant enzyme to find a new cancer treatment approach. Chem. 2017; 398 (10): 1095–1108.
  6. Hrycay EG, Bandiera SM. Involvement of Cytochrome P450 in Reactive Oxygen Species Formation and Cancer. Pharmacol. 2015; 74: 35–84.
  7. Ibuki FK, Bergamaschi CT, da Silva Pedrosa M, Nogueira FN. Effect of vitamin C and E on oxidative stress and antioxidant system in the salivary glands of STZ-induced diabetic rats. Oral. Biol. 2020; 116: 104765.
  8. Jiang Q. Natural forms of vitamin E as effective agents for cancer prevention and therapy. Adv. Nutr. 2017; 8 (6): 850–867.
  9. Korolyuk MA, Ivanova LI, Mayorova IG, Tokareva VE. Method for determination of catalase activity. Lab 1988; 1: 16–19. (in Russian)
  10. Moloney JN, Cotter TG. ROS signalling in the biology of cancer. Cell Dev. Biol. 2018; 80: 50–64.
  11. Morris G, Anderson G, Dean O, Berk M, Galecki P, Martin-Subero M, Maes M. The glutathione system: a new drug target in neuroimmune disorders. Neurobiol. 2014; 50 (3): 1059–1084.
  12. Mumic FT, Silveira MRG, Vilalva KH, Jordani ME, Gomes MCJ, Vanni JC, Vollet Filho JD, Kurachi C, Bagnato VS, Silva OC. Effect of irradiation with different laser wavelengths on oxidative stress of non-hepatectomized rats. Acta Cir. Bras. 2016; 31 (1): 40–44.
  13. Pawlowska E, Szczepanska J, Blasiak J. Pro- and antioxidant effects of vitamin C in cancer in correspondence to its dietary and pharmacological concentrations. Med. Cell Longev. 2019; 2019: 7286737.
  14. Schenkman JB, Cinti DL. Preparation of microsomes with Calcium. Enzymol.1978; 52 (C): 83–89.
  15. Sheng Y, Abreu IA, Cabelli DE, Maroney MJ, Miller AF, Teixeira M, Valentine JS. Superoxide dismutases and superoxide reductases. Rev. 2014; 114 (7): 3854–3918.
  16. Sirota TV. Novel approach to the study of adrenaline auto-oxidation and its use for the measurements of superoxide dismutase activity. Med. Khim. 1999; 45 (3): 109–116. PMID: 10432564. (in Russian)
  17. Trineeva OV. Methods of analysis of vitamin E. VSU. Chem., Biol., Pharm. Series. 2013; 1: 212–224. Available at: (in Russian)
  18. Vlasova SN, Shabunina EI, Pereslegina I. A. The activity of the glutathione-dependent enzymes of erythrocytes in chronic liver diseases in children. Lab. Delo. 1990; 8: 19–22. PMID: 1705592. (in Russian)
  19. Weinbach EC. A procedure for isolating stable mitochondria from rat liver and kidney. Biochem. 1961; 2 (4): 335–343.
  20. Yang CS, Luo P, Zeng Z, Wang H, Malafa M, Suh N. Vitamin E and cancer prevention: studies with different forms of tocopherols and tocotrienols. Carcinogen. 2020; 59 (4): 365–389.




WorldCat Logo