Bìol. Tvarin. 2020; 22(1): 20–25.
Received 16.09.2019 ▪ Accepted 18.03.2020 ▪ Published online 01.05.2020

Effects of copper nanoparticles on the biochemical parameters of blood serum of female rabbits

Yu. I.  Slyvchyk1, O. V. Shtapenko1, I. I.  Hevkan1, V. Y. Syrvatka2, O. Yu. Slyvchyk3, A. R. Korbetsky1
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1Institute of Animal Biology NAAS,
38 V. Stusa str., Lviv, 79034, Ukraine, This email address is being protected from spambots. You need JavaScript enabled to view it.

2Lviv National University named after Ivan Franko,
4 Hrushevskoho str., Lviv, 79005, Ukraine

3State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives,
11 Donetska str., Lviv, 79019, Ukraine

This article presents the results of physiological and biochemical investigations of the copper nanoparticles effect on the organism of mature female rabbits. Clinical observations and biochemical parameters of blood would permit to assess the safety of use of obtained nanoparticles and to evaluate the influence of prolonged intraperitoneal administration thereof on the metabolism of the animals, as well as, basing on further studies, to develop drugs for the treatment of various pathologies of the reproductive system of farm animals. For this investigation 18 four-month-old female rabbits of the Blanc de Termonde breed weighing 3–3.5 kg and analogous by biochemical parameters of blood serum were selected. They were divided into three groups: control, 1st and 2nd experimental ones, each group containing 6 rabbits. Every 7th day of the 28-day period the rabbits were subjected to intraperitoneal administration of drugs. The rabbits of the control group received 0.9 % physiological solution as placebo, 1.5 ml/kg per injection. The rabbits of the experimental group received a preparation containing copper nanoparticles. The investigation results showed a positive influence of the studied copper nanoparticles on the metabolic processes. Intraperitoneal administration of copper nanoparticles on initial stages of the study (after the second injection) produced increased ALT and AST activities, which might be associated with the adaptation of organism to the introduced drugs. The decreased LDH activity in the blood serum of the rabbits during the administration of the preparations might be associated with enhanced intracellular metabolic processes requiring considerable expenditures of energy. The dynamics of the gamma glutamate transferase activity remained on the same level in the control and both experimental groups of female rabbits, indicating that the introduced copper nanoparticles drugs were not toxic. The administration of copper nanoparticles in the dose of 50 μg/kg of body weight has positive effects on the reproductive system of female rabbits and the processes of embryonic development.

Key words: rabbits, copper nanoparticles, enzymes, implantation, corpus luteum

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