Bìol. Tvarin, 2018, volume 20, issue 3, pp. 84–92

CONTENT OF LIPIDS IN TISSUES OF THE MELLIFEROUS BEES ADDITIONAL FED WITH SOYBEAN MEAL, SUGAR SYRUP AND CITRATES OF Co AND Ni

L. I. Romaniv, I. I. Kovalchuk, A. G. Pashchenko, R. S. Fedoruk

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Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

The article presents the research results of the influence of addition to melliferous bees sugar syrup (SS), SS and soybean flour (SF) (II) and SS, SF and Co and Ni citrates (СoNC + NiNC) in a dose of 2 and 1 mg separately (III, IV) and in combination (V group) (the 1st stage) with the preservation of this scheme for the I and II groups on the 2nd stage the exclusion of soybeans in III, IV and V groups I with their placement into entomological gardens on the lipid metabolism indices in the homogenate of tissues.

The research results did not show probable differences (1st stage) in the crude lipids (CL) in the tissues of melliferous bees of the II, III, IV and V experimental groups compared with the control group. Similar situation without probable changes in the content of crude lipids in the tissues of the II, III, IV and V experimental groups maintained at the second stage of the research without the addition of soybean flour. More pronounced differences were established in the correlation of individual lipids classes in the tissues of bees organism of all experimental groups compared with control. Additional feeding of soy flour, sugar syrup and Co and Ni citrates causes a significant increase in the content of phospholipids in the samples of bees tissues of the III, IV and V groups; mono- and diacylglycerols (in II, III and IV), and cholesterol esters — only in the II and III experimental groups on the background of a probable decrease in the triacylglycerol content in the II, III, IV and V experimental groups compared with their content in the tissues samples of the control (I) group. Separate addition of Co and Ni citrates in doses of 2 and 1 mg in accordance with 25 ml of 50 % sugar syrup to the bees of III and IV experimental groups and their combination  2 mg of Co + 1 mg of Ni with 25 ml (50 %) of sugar syrup in V group determines the probable increase in the phospholipids content in the tissues of bees of the III, IV and V groups compared with the control group (I), NEFA, mono and diacylglycerols in the II, III and IV experimental groups. However, under the action of these doses of Co and Ni citrates without soy flour on the second stage of the research in tissues of the organism of bees II, III and IV, there was a probable decrease in the content of cholesterol esters and triacylglycerols (similar to the first stage) with the increase of their level only in bees of the V group. A decrease was marked in the content of free cholesterol in the tissues of V group the bees and its increase in the III and IV experimental groups compared to the control.

Keywords: BEES, TISSUES, LIPIDS, SOYBEAN, SUGAR SYRUP, COBALT AND NICKEL CITRATES, ENTOMOPHILIC GARDENS

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