Bìol. Tvarin, 2018, volume 20, issue 3, pp. 37–42


O. I. Koleshchuk, I. I. Kovalchuk, M. M. Tsap, M. I. Khrabko

This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Animal Biology NAAS,
38 V. Stusa str., Lviv 79034, Ukraine

The article presents experimental data on the influence of nickel citrate obtained using nanotechnology on immunobiological parameters and antioxidant protection in the body of cows. It has been established that inclusion of nickel citrate in the amount of 0.1 mg/kg s.p. feed (II group) and 0.3 mg/kg s.p. feed (group III) in the diet of cows on the 9th month of calving and in the first two months after calving positively influenced the functioning of the antioxidant system and the immunobiological parameters of the organism of animals with certain differences. During the first month of feeding in blood of animals of the II group, an increase in the activity of catalase was observed at 11.18 % and SOD at 28 %. In the blood of animals of the III group, there was a slight increase in the activity of catalase by 4.9 % and an increase in the activity of SOD by 18.37 %. During the 2nd month of feeding, there was an increase in the activity of glutathione peroxidase in the blood of animals of both groups by 10.38 % and 9.91 %, respectively, as well as increase of activity of SOD at 25.25 % in the blood of animals of ІІІ group. Nutrition of cats with Ni citrate for 2 months after calving resulted in a probable decrease in the HPL content: in the blood of cows of the II group by 4.2 %, and in cows of III group by 2.4 %. There was also a tendency towards a decrease in the content of TBK-active products in the blood, which is a terminal metabolite of LPO. Feeding to cows of 0.1 mg/kg nickel citrate (II group) contributed to a probable decrease in the content of circulating immune complexes in their blood serum by 19.2 %, and in ІІ group which was fed 0.3 mg/kg s. feeding stuffs — by 18.9 %.


  1. Bartosz G. Reactive oxygen species: Destroyers or messengers? Biochemical Pharmacology, 2009, vol. 77, issue 8, pp. 1303–1315. https://doi.org/10.1016/j.bcp.2008.11.009
  2. Brucka-Jastrzębska E., Protasowicki M. Elimination dynamics of nickel, administered by a single intraperitonial injection, in common carp, Cyprinus carpioL. Acta Ichthyologica et Piscatoria, 2004, vol. 34, no. 2, pp. 181–192. https://doi.org/10.3750/AIP2004.34.2.06
  3. Djordjević V. B. Free radicals in cell biology. International Review of Cytology, 2004, vol. 237, pp. 57–89. https://doi.org/10.1016/S0074-7696(04)37002-6
  4. Fedoruk R. S., Kravtsiv R. Y. Physiological mechanisms of adaptation of animals to environmental conditions. The Animal biology, 2003, vol. 5, no. 1–2, pp. 75–82.
  5. Forgács Z., Némethy Z., Révész C., Lázár P. Specific amino acids moderate the effects on Ni2+ on the testosterone production of mouse leydig cells in vitro. Toxicol. Environ. Health A, 2001, vol. 62, issue 5, pp. 349–358. https://doi.org/10.1080/152873901300018075
  6. Hostynek J. J. Sensitization to nickel: etiology, epidemiology, immune reactions, prevention, and therapy. Rev. Environ. Health, 2006, vol. 21, issue 4, pp. 253–280. https://doi.org/10.1515/REVEH.2006.21.4.253
  7. Kaplunenko V. G., Kosinov N. V., Polyakova D. V. Getting new nutrients and biocidal nanomaterials using erosion-explosive dispersion of metals: Proceedings of the Materials Research and practical conference with internationalparticipation “Nanotechnology and nanomaterials in biology and medicine”, Novosibirsk, SibUPK, 2007, pp. 134-137. (in Russian)
  8. Khomyn M. M., Fedoruk R. S., Kropyvka S. Y. Biochemical processes in the cows and biological value of milk under the influence of citrate chromium, selenium, cobalt and zinc. The Animal biology, 2015, vol. 17, no. 1, pp. 155–162. (in Ukrainian)
  9. Kurtyak B. M. Characteristics of the metabolism of cows in the pre- and postpartum periods of cows and the role of vitamins A, D, E and selenium in its correction: author’s abstract. Dr. veterinary sci. diss., Lviv, 2006, 296 p. (in Ukrainian)
  10. Murlooney S. B., Hausinger R. P. Nickel uptake and utilization by microorganisms. FEMS Microbiology Reviews, 2003, vol. 27, issue 2–3, pp. 239–261. https://doi.org/10.1016/S0168-6445(03)00042-1
  11. Sitar O. V., Novitsky N. V., Taran N. Yu. Nanotechnology in modern agriculture. Physics of living, 2010, vol. 18, pp. 113–116. (in Ukrainian)
  12. Valko M., Morris H., Cronin M. T. Metals, toxicity and oxidative stress. Curr. Med. Chem., 2005, vol. 12, issue 10, pp. 1161–1208. https://doi.org/10.2174/0929867053764635

Download full text in PDF




WorldCat Logo