Bìol. Tvarin, 2018, volume 20, issue 3, pp. 16–23


AZDychok, Ya. V. Lesyk, M. M. Tsap

This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Animal Biology, NAAS,
38 V. Stus str., Lviv 79034, Ukraine

The article presents the results of the study of the influence of the production of rabbits from different periods of 60 to 118 days of various amounts of sulfur citrate, obtained by the method using nanotechnology and sodium sulfate, on the parameters of cellular and humoral immunity in the body. The research has established the probable differences between the experimental and control groups based on the relative content of phagocytic activity of neutrophils in the blood of rabbits, which were given silicate citrate at the rate of 4 and 8 S/kg body weight, indicating the stimulatory effect of the organic compound sulfur on the cellular level of non-specific resistance of their organism.

The expression of animals of experimental groups of sulfur citrate, in comparison with sodium sulfate and control group, more strongly influenced the indices of non-specific resistance of the organism of humoral type, which was higher (P<0.05) relative content of lysozyme and bactericidal activity of serum of rabbits of the III experimental group during the study and the second group on the 58th day of the experiment, which may indicate a positive effect of a separate amount of sulfur citrate on the course of metabolic processes involved in the formation of humoral mechanisms for body. The highest (P<0.05–0.01) content of hexoses bound to proteins and sialic acids in the blood of animals of the I−III experimental groups was obtained, which was expressed as sulfate citrate, which was more expressed on the 58th day of the experiment. An increase in the content of glycoproteins and their carbohydrate components in the blood within the limits of physiological values indicates an increase in the body’s resistance to the effects of individual amounts of sulfur citrate.

The release of an organic sulfur additive in the rabbit diet has a stimulating effect on the functioning of the immune system of their organism, which led to a possible increase in the content of immunoglobulins in the blood of rabbits II experimental group on 58th day of the study and II and III groups in the 31st day of the experiment compared with the control group, indicating activation of the immune response of the organism to the action of the applied amounts of citrate sulfur.


  1. Ashwell C., Harpord J. Carbohydrate-Specific Receptors of the Liver. Annual Review of Biochemistry., 1982, vol. 51, pp. 531–554. https://doi.org/10.1146/annurev.bi.51.070182.002531
  2. Azarenyuk L. S, Generalov I.Y. New functions of antibodies: Review. Teron. archiv, 1990, no. 5, pp. 149–153. (in Ukrainian).
  3. Borysevych V. B., Kaplunenko V. G., Kosinov M. V. Nanomaterials in biology. Fundamentals of nanoveterinary. A textbook for veterinary students and for veterinary and medical specialists. Kyiv, Avicenna Publ., 2010, p. 416. (in Ukrainian)
  4. Carlos de Blas, Wiseman J. Nutrition of the Rabbit. 2nd Ed. Library of Congress Cataloging-in-Publication Data, 2010, p. 325.
  5. Chekman I. S., Ulberg Z. R., Malanchuk V. O. Nanoscience, nanobiology, nanopharmaceutics. Kyiv, Poligraphplus, 2012, p. 328. (in Ukrainian)
  6. Eliseev A. A., Lukashin A. V. Functional Nanomaterials. Moscow, Fizmatlit, 2010, p. 456. (in Russian)
  7. Fortina P., Kricka L. J., Surrey S., Grodzinski P. Nanobiotechnology the promise and reality of new approaches to molecular recognition. Trends in Biotechnology, 2005, vol. 23, issue 4, pp. 168–173. (in Ukrainian) https://doi.org/10.1016/j.tibtech.2005.02.007
  8. Kazimirko V. Antioxidant system and its functioning in the human body. Health of Ukraine, 2004, no. 98, pp. 155–175. (in Ukrainian)
  9. Klitsenko H. T., Kulyk M. F., Kosenko M. V., Lisovenko V. T. Mineral nutrition of animals. Kyiv, Svit, 2001, 575 p. (in Ukrainian)
  10. Knopp D., Tang D., Niessner R. A review: Bioanalytical applications of biomolecule-functionalized nanometer-sized doped silica particles. Analytica Chimica Acta, 2009, vol. 647, issue 1, pp. 14–30. https://doi.org/10.1016/j.aca.2009.05.037
  11. Lagoduk P. Z., Gren R. J. Growth, development and protein metabolism in the body of repair young animals with sodium sulfate added to feed. Agricultural Biology, 1988, no. 4, pp. 124–130. (in Ukrainian)
  12. Logan H. M., Cathala N., Grignon C., Davidian J. C. Cloning of a cDNA encoded by a member of the Arabidopsis thaliana ATP sulfurylase multigene family. Expression studies in yeast. J. Biol. Chem., 1996, vol. 271, no. 21, pp. 12227–12233. https://doi.org/10.1074/jbc.271.21.12227
  13. Mueller J. W, Shafqat N. Adenosine-5’-phosphosulfate — a multifaceted modulator of bifunctional 3’-phospho-adenosine-5’-phosphosulfate synthases and related enzymes. FEBS J., vol. 280, issue 13, pp. 3050–3057. https://doi.org/10.1111/febs.12252
  14. Murthy S. K. Nanoparticles in modern medicine: state of the art and future challenges. Int. J. Nanomed., 2007, vol. 2, issue 2, pp. 129–141.
  15. Nagoyev B. S. Reference book on immunology. Nolchik, Elbrus, 2002, p. 192.
  16. Official Journal of the European Union L276/33. Directive 2010/63/EU of The European Parliament and of The Council of 22 September 2010 on the protection of animals used for scientific purposes. 86/609/EC. 20.10.2010.
  17. Ratych I. B. Biological role of sulfur and metabolism of sulfate in poultry. Lviv, 1992, p. 170.
  18. Skalny A. V., Rudakov I. A. Bioelements in medicine. Publishing House, World, 2004, p. 272. (in Russian)
  19. Schwartz N.B., Lyle S., Ozeran J. D., Li H., Deyrup A., Ng K., Westley J. Sulfate activation and transport in mammals: system components and mechanisms. Chem. Biol. Interact., 1998, vol. 109, issue 1–3, pp. 143–151. https://doi.org/10.1016/S0009-2797(97)00129-4
  20. Shimoike T., Inoguchi T., Umeda F., Nawata H., Kawano K., Ochi H. The meaning of serum levels of advanced glycosylation end products in diabetic nephropathy Metabolism, 2000, vol. 49, issue 8, pp. 1030–1035. https://doi.org/10.1053/meta.2000.7738
  21. Sedilo G. M, Makar I. A, Gavrylyak V. V., Gumenyuk V. V. A monograph. Lviv, PAIS, 2009, p. 148. (in Ukrainian)
  22. Trachtenberg I. M., Chekman I. S., Linnik V. O., Kaplunenko V. G. Interaction micronutrients, biological, medical and socialaspects. Bulletin of the National Academy of Sciences, 2013, vol. 6, pp. 11–20. (in Ukrainian)
  23. Tsuboi S., Fukuda M. Mammary gland. biol. neoplasia, 2001, vol. 6, no. 3, pp. 355–364.
  24. Varki A., Freeze H. Subcellular biochemistry. Membrane Biogenesis, 1994, pp. 71–100.
  25. Voronin E. S., Petrov A. M. Immunology. Koloss-press, 2002, p. 230.
  26. Volchegorsky I. A., Tyshevskaya N. V., Kuznetsov D. A. Effect of “medium molecules” isolated from blood plasma of intact and burned animals, on cellular composition of cultures of erythroblastic islets of bone marrow. Bulletin of the Russian Academy of Medical Sciences, 2002, no. 2, pp. 134–147.
  27. Vlizlo V. V. (ed.), Fedoruk R. S., Ratych I. B. Laboratory methods of investigation in biology, stock-breeding and veterinary medicine. A reference book. Lviv, Spolom, 2012, 764 p. (in Ukrainian)
  28. Yanovych V. H., Solohub L. I. Biological bases of transformation of nutrients in ruminants. 2002, 384 p. (in Ukrainian)

Download full text in PDF




WorldCat Logo