Bìol. Tvarin, 2018, volume 20, issue 1, pp. 60–69


І. Matiukha1, O. Vischur1, N. Broda1, D. Mudrak1, G. Sobko2
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1Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

2Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytsky,
50 Pekarska str., Lviv 79010, Ukraine

Research and evaluation of new drug on natural basis in cows with subclinical form of mastitis was conducted. In our investigations we proved significantly positive effects “Antimast” both in the form of injections and “Antimast” ointment on common state of cows with clinical mastitis and on regulation of pro- and antioxidant balance in the organism during treatment.
In this article the action of the injection drug “Antimast” and “Antimast” ointment was established by analyzing the peroxides indexes and antioxidant capabilities in the blood and milk of the bovine cattle. Increasing the intensity of peroxidation reactions as well as lower antioxidant activity in the blood and milk of cows with subclinical mastitis has been discovered. The mean levels of TBARS and lipid hydroperoxides was higher in dairy cows with acute subclinical mastitis compared to the normal indexes in healthy animals indicating a worse state of oxidative stress. After the injection of experimental drug, normalization of the content of oxidative products and at the same time activation of antioxidant enzymes in the blood and milk of the cows were revealed. The same but less pronounced effects of ointment were found.
Both types of “Antimast” played role as activators of endogenic defense system, moreover injection of “Antimast” intracysternaly leads to significant intensively growth of both enzymes compare to ointment action. Activity of SOD after injection was 23.15±0.89 (U·mg1 protein) compare to 22.45±1.08 (U·mg1 protein) after ointment on the 9th day of treatment.


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