Bìol. Tvarin, 2018, volume 20, issue 1, pp. 49–53


О. Zastulka
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National University of Life and Environmental Sciences of Ukraine,
15 Heroiv Oborony str., Kyiv 03041, Ukraine

The article describes different ways of reducing the contamination of microscopic fungi in bee pollen, that had qualitative physicochemical indices regarding water content, flavonoids content, active acidity and the content of crude protein according to the national standard 3127-95, but with 2.5–10 times high content of microscopic fungi.
Mycological research was carried out on Sabouraud agar. The method of mycological research of experimental (treated) and native (raw) samples consisted in the fact that the collected 1 g of bee pollen was placed in a flask, where 0.9 % NaCl solution was added and stirred for 1020 min. Than we aseptically transferred 1 cm3 of suspension in Petri dishes, poured molten and cooled agar, carefully mixed the suspension with the nutrient medium in circular motions. At the same time, a study of 6 samples of bee pollen was conducted using freezing, infrared irradiation and honey dilution for recording microscopic fungi.
Grinding on an electric mill, freezing and defrosting 3 times of bee pollen do not allow reducing substantially the content of microscopic fungi in the forehead. Dilution of 10 times by honey in bee pollen significantly (P<0.01) reduces the content of fungi by 7.9 times, and 20 times dilution with honey causes a significant (P<0.01) decrease in microscopic fungi by 13.94 times. In case of contamination of the bee pollen with microscopic fungi by the genera Mucor and Rhizopus and the absence of contamination by Aspergillus genera, its safety can be increased by exposing infrared rays for 1 hour or by making 10 % honey-pollen product for use by humans or animals. It is necessary to conduct mycological control of bee pollen, if using bee pollen for the manufacture of candy for bees and honey-pollen products for people. If, however, the bee pollen is contaminated by fungi of the genus Aspergillus, it should be subjected to an additional study on the possibility of toxin formation.


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