Bìol. Tvarin, 2015, volume 17, issue 3, pp. 104–109

EFFECT OF TAURINE ADMINISTRATION ON ACTIVITY OF SUPEROXIDEDISMUTASE IN RAT TISSUES

D. R. Ostapiv1,2, V. V. Manko1

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1Ivan Franko National University of Lviv,
4 Hrushevskyi str., Lviv 79005, Ukraine

2SSRCI of Veterinary Medicinal Products and Feed Additives,
11 Donetska str., Lviv 79019, Ukraine

Aim of work was to study the activity of superoxide dismutase and its isozymes in rat tissues under the conditions of prolonged oral administration of taurine. Male Wistar rats weighing 190–220 g and age 4.5 months, were divided into three groups. Rats for 28 days, daily, though esophagus probe were injected: control — drinking water and two experimantal — taurine solution of 40 (I experimental group) and 100 (II experimental group) mg/kg, respectively. After completing the research animals were decapitated under light chloroform anesthesia and liver, brain, testis and thigh muscle tissues were isolated. Tissues were homogenized, centrifuged. In supernatant was determined total superoxide dismutase and isozyme content after electrophoresis in 10 % polyacrylamide gels, staining with tetrazolium nitroblue. Given the percentage isozyme, the activity of each was deducted from the total activity of the enzyme.

It was found that activity of brain superoxide dismutase remains within control under the conditions of prolonged per oral administration of taurine. However, cytoplasmic redistribution of isozyme activities occurs — S2 increases, and S3 and S1 decreases. The superoxide dismutase liver of I experimental group animals is the highest. This occurs because of the increase in activity of extracellular (S5) and cytoplasmic (S2) isozymes. With the increase of SOD activity in testes of II experimental group increases the quantity and value of all isozymes. In muscles of both experimental groups increased total activity, with it grow extracellular (S5) and cytoplasmic (S1) activity of superoxide dismutase isozymes.

Consequently, the impact of taurine long-term oral administration on activity of superoxide dismutase is realized through isozyme content change, and is tissue- and dosespecific.

Keywords: TAURINE, SUPEROXIDE DISMUTASE, ISOZYME, LIVER, BRAIN, TESTES, THIGH MUSCLE

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