Bìol. Tvarin, 2015, volume 17, issue 3, pp. 57–62

RESEARCH OF THE EQUINE REPRODUCTIVE SYSTEM MICROFLORA

M. Krivda, A. Galatuk, L. Solodka

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Zhytomyr National Agroecological University,
Staryi bulvar st., 7, Zhytomyr, 10009, Ukraine

Research of the microbial cells amount and species of composition in the biofilms is a progressive direction nowadays. It became studied not only by veterinary microbiologists, but experts of humane medicine. Scientists from all the world proved that quantitative and qualitative disturbances of microbial consortium can lead to the pathological process of an animal or humans organism systems (respiratory tract, gastrointestinal tract, reproductive system and so on). The goal of our research was to study the microbial landscape of the mare’s genital tract. This article presents the results of the study of potential pathogens — facultative anaerobic colonizers of horse reproductive tract.

Comparison of the spectrum of microbes isolated from swabs of genital system of healthy mares and animals with deflexion from well being allowed to establish the nature of microbial contamination of reproductive tract epithelium. Comparing the microbe’s spectrum isolated from swabs of the genital system of clinically healthy mares and individuals with pathologies showed prevalence of rod-shaped organisms in clinically healthy animals (57 %), while in mares with deregulation this trend was not always preserved (44–55 %). The analysis of the microorganisms, isolated from cervical of mares, was particularly informative. So overall contamination of the uterus cervix in clinically healthy mares was 2–6,5 times less than in animals in which deregulation had been observed. For visualisation of qualitative diversity of the composition of mare’s reproductive system biofilm the cultural and morphological qualities of extracted microorganisms were analyzed. These microorganisms were assigned to such groups as coccus bacteria asporogenous rod bacteria, bacillus and actinomycetes.

After studying the intensity (speed) of microbial growth, cultural and morphological characteristics their sensitivity to antimicrobial medications of different pharmacological groups was investigated. This information will be useful in case of clinical symptoms of dysbiosis for optimizing animal treatment.

Keywords: EQUINE, MICROBIOLOGICAL ASSESSMENT, CONTAMINATION, BACTERIAL CONTAMINATION, REPRODUCTIVE SYSTEM, MORPHOLOGICAL GROUP, ANTIBIOTIC SENSITIVITY

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