Bìol. Tvarin, 2017, Volume 19, Issue 2, pp. 16–22

EXPRESSION OF IMUNORECEPTOR PROTEINS IN PLASMOLEMMA OF ENTEROCYTES IN FORMATION OF COLOSTRIC IMMUNITY IN NEWBORN CALVES

SIGolopura, MOMarinyuk, MITsvilikhovsky

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National university of life and environmental sciences of Ukraine,
16 Polkovnika Potekhina str., Kyiv 03041, Ukraine

Indexes of content of proteins with molecular masses of 37, 40 and 43 kDa in plasmolemma of small intestine enterocytes in newborn calves in dynamics from the birth till the age of 24 hours were investigated. The research was conducted on two groups (control and research) of the newborn calves of Ukrainian black-and-white breed; the samples of jejunum enterocytes were taken and the content of proteins from membrane fraction of lysates was isolated. The relatively high content of expression of proteins with М? of 37, 40 and 43?kD ? in membrane fraction of lysates of enterocytes from jejunum of just born calves of control group with further significant decreasing of total content of these proteins at the age of 6 hours and with their following increasing at the age of 24 hours comparatively with the previous one was shown.

М of 37, 40 and 43 kD а in membrane fraction of lysates of enterocytes from jejunum of just born calves of control group with further significant decreasing of total content of these proteins at the age of 6 hours and with their following increasing at the age of 24 hours comparatively with the previous one was shown.

The application of macrocapsules from phospholipid bilayer on the basis of soy-bean lecithin for newborn calves activates the Ig transport in small intestine during the period of colostral immunity formation that assists the increasing of its level. The total content of proteins with MM of 37, 40 and 43 kDa in plasmolemma of jejunum enterocytes in calves of the research group at the age of 6 hours significantly decreased with its further increasing at the age of 24 hours compared to these indexes in calves from control group. The dynamics with comparative analysis between fractions of expression of proteins with MM of 37, 40, and 43 kDa in plasmolemma of jejunum enterocytes in the newborn calves from control and research group is shown. The application of macrocapsular preparation comparing to the control group indicates more intensive level of recycling of receptor proteins through enterocytes from BM to AM with further reception of colostrum Ig and their transcellular transportation.

Keywords: NEWBORN CALVES, SMALL INTESTINE, ENTEROCYTE, PLASMOLEMMA, PROTEINS, PHOSPHOLIPID, MACROCAPSULES, COLOSTRIC IMMUNITY

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