Bìol. Tvarin, 2019, volume 21, issue 3, pp. 79–86

DYNAMICS OF NON-SPECIFIC RESISTANCE IN PIGS AFTER ACTION OF NEW IMMUNOTROPIC DRUGS

S. I. Fedoryshyn, I. O. Matiukha, N. A. Broda, D. I. Mudrak

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Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

The article presents the results of research on the influence of new immunotropic drugs containing piperidine acetate on the status of the cellular and humoral links of the non-specific resistance in piglets during the process of their cultivation. A comparative analysis of the influence of “Trifuzol” drugs and a new immunotropic agent in the form of a liposomal emulsion “Nero” on the dynamics of indicators of the natural resistance of the piglet organism is carried out. The experiment was conducted in three groups of pigs, analogues by body weight and sex, for 4 animals in each group. For pigs on the 2nd and 14th day of age, an intramuscular dose of 1 ml per animal was introduced: 1st experimental group — Trifuzol 1 %; control group — isotonic solution; the 2nd experimental group — the new liposomal drug “Nero”. Material for research was the blood of piglets at 2-, 7-, 14- and 26-day-old age. The blood serum was used to determine the bactericidal and lysozyme activity (BA and LA), as well as the content of circulating immune complexes (CIC) and middle mass molecules (AWM); in the blood — phagocytic activity, index and number. In pigs, which were treated by liposomal preparation at the 14th and 26th day of life the higher phagocytic activity (PhA) of blood neutrophils was confirmed. Significant changes in the humoral level of non-specific resistance have been found in the pigs for the investigational drugs, as evidenced by a significantly higher bactericidal (BA) and lysozyme activity of the serum (LA), both in terms of the action of the drug Trifuzol and liposomal drag. Developed liposomal preparation “Nero” caused a reliable increase in the intensity of BASK in piglets from the 7th day of life and until the end of the experiment, and lysozyme activity at 26-day age was 22 % (P<0.01) higher than in control. The applied drugs contributed to a gradual decrease in the concentration of circulating immune complexes (CIC) in the blood of pigs, especially these changes were expressed at 14- and 26-day-old age. In the age dynamics, there was an increase in the content of medium-weight molecules (MSM) in piglets’ blood. The introduction of the liposomal preparation caused a probable decrease in their content in the piglets’ blood at 26 days of age, indicating the inhibitory effect of its factors on the content of products of endogenous intoxication of the body.

Keywords: PIGLETS, BLOOD, HUMORAL FACTORS OF PROTECTION, BACTERICIDAL ACTIVITY, PHAGOCYTAL ACTIVITY, LYSOCIMIC ACTIVITY, IMMUNOMODULATORS

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