Bìol. Tvarin, 2019, volume 21, issue 3, pp. 33–41

FEATURES OF FUNCTIONING OF THE REPRODUCTIVE SYSTEM OF HONEY BEES QUEEN AFTER FEEDING THE SILVER AND COPPER NANOPARTICLES CITRATE-BASED

I. I. Dvylyuk1, I. I. Kovalchuk1, I. V. Dvylyuk2

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1Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

2Lviv National University of Veterinary Medicine and Biotechnologies named after S. Gzhytsky,
50 Pekarska str., Lviv 79010, Ukraine

The paper reviews some aspects of the stimulating feeding influence on the functional activity of the reproductive system in honeybee queens. The purpose of the research was to study the peculiarities of functioning of the reproductive system in the honeybee queens under the conditions of feeding the silver and copper citrates obtained by the nanotechnology method. Bees of the control group were kept in stationary conditions with sugar syrup feeding (1000 ml/week/bee colony). Bees of the experimental group were kept in the same conditions and were fed with sugar syrup containing the addition of 0.5 mg silver and 0.5 mg copper nanoparticles citrate-based. A photometry of a capped brood, a histological examination of the ovary of the honeybee queen, an optical microscopy of histological sections, weighing and morphometry of 12-hour eggs obtained from worker bee cells was carried out. It has been established that the intensity of the average daily oviposition in the experimental group of the honeybee queens increased in comparison with the control to 21.25 %. An increase in the average weight of eggs for 1.4 %, 2.9 % and 5.9 % in the experimental group was detected, respectively, in the II, III and IV period of the study compared to the control. There was an increased activity in ovarian germ zones in queen of experimental group. In the experimental group, an increased intensity of trophoblastic activity was observed. Also in the control group, the development of apoptosis of trophocytes in the early stages of oogenesis and the appearance of destructive changes in oocytes was observed. Against the background increase of the average weight of eggs obtained from the bees of the experimental group, the histological analysis of ovarian sections indicates an intensified oogenesis in the bee mothers of the experimental group as compared to the control one, where there were signs of oppression of oogenesis.

Keywords: HONEY BEE, BEE QUEENS, REPRODUCTIVE SYSTEM, OOGENESIS, SILVER AND COPPER NANOPARTICLES CITRATE-BASED

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