Bìol. Tvarin, 2019, volume 21, issue 3, pp. 33–41


I. I. Dvylyuk1, I. I. Kovalchuk1, I. V. Dvylyuk2

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1Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

2Lviv National University of Veterinary Medicine and Biotechnologies named after S. Gzhytsky,
50 Pekarska str., Lviv 79010, Ukraine

The paper reviews some aspects of the stimulating feeding influence on the functional activity of the reproductive system in honeybee queens. The purpose of the research was to study the peculiarities of functioning of the reproductive system in the honeybee queens under the conditions of feeding the silver and copper citrates obtained by the nanotechnology method. Bees of the control group were kept in stationary conditions with sugar syrup feeding (1000 ml/week/bee colony). Bees of the experimental group were kept in the same conditions and were fed with sugar syrup containing the addition of 0.5 mg silver and 0.5 mg copper nanoparticles citrate-based. A photometry of a capped brood, a histological examination of the ovary of the honeybee queen, an optical microscopy of histological sections, weighing and morphometry of 12-hour eggs obtained from worker bee cells was carried out. It has been established that the intensity of the average daily oviposition in the experimental group of the honeybee queens increased in comparison with the control to 21.25 %. An increase in the average weight of eggs for 1.4 %, 2.9 % and 5.9 % in the experimental group was detected, respectively, in the II, III and IV period of the study compared to the control. There was an increased activity in ovarian germ zones in queen of experimental group. In the experimental group, an increased intensity of trophoblastic activity was observed. Also in the control group, the development of apoptosis of trophocytes in the early stages of oogenesis and the appearance of destructive changes in oocytes was observed. Against the background increase of the average weight of eggs obtained from the bees of the experimental group, the histological analysis of ovarian sections indicates an intensified oogenesis in the bee mothers of the experimental group as compared to the control one, where there were signs of oppression of oogenesis.


  1. Barbosa P., Berry D. L., Kary Ch. S. Insect Histology: Practical Laboratory Techniques. Wiley blackwell, 2015, 380 p. DOI: 10.1002/9781118876114. (in Ukrainian)
  2. Brandorf A. Z., Ivoylova M. M. Oviposition of queens in the assessment of honey productivity and winter hardiness of honey bee colony. Beekeeping, 2012, issue 6, pp. 16–18. (in Russian)
  3. Dvylyuk I. V, Kovalchuk I. I., Dvyluk I. I., Chervinka K. A. Method of photometry of sealed breeder of bees. Patent UA no. 122394, 2018. (in Ukrainian)
  4. Fedorov I. Silver nanoparticles. Newsletter of innovations, 2005, vol. 1 (2), pp. 25–31. (in Russian)
  5. Glushchenko N., Bohoslovskaya O., Olkhovskaya I. Comparative toxicity of salts and nanoparticles of metals and the peculiarity of their biological action. Proceeding of the International scientific and practical conference “Nanotechnology and information technologies — technologies of the 21st century”, Moscow, 2006, pp. 93–95. (in Russian)
  6. Nemova T. Prospects of application chemical agents on the basis of nanotechnology in veterinary medicine. Veterinary medicine of Ukraine, 2013, issue 1, pp. 35–37. (in Ukrainian)
  7. Pestovsky Y. S., Martínez-Antonio A. The use of nanoparticles and nanoformulations in agriculture. Journal of Nanoscience and Nanotechnology, 2017, vol. 17, issue 12, pp. 8699–8730. DOI: 10.1166/jnn.2017.15041.
  8. Pishak V., Fedonyuk L. Ya., Fedonyuk V. V. Histology with the basics of histological technology. Kyiv, Kondor, 2008, 400 p. (in Ukrainian)
  9. Rangel J., Keller J. J., Tarpy D. R. The effects of honey bee (Apis mellifera) queen reproductive potential on colony grows. Insectes Sociaux, 2013, vol. 60, pp. 65–73. DOI: 10.1007/s00040-012-0267-1.
  10. Romais B. Microscopic technique. Moscow, Publishing House of Foreign Literature, 1953, 718 p. (in Russian)
  11. Savenkova O. Morphological aspects of the influence of nanoproducts on an organism. Bulletin of problems of biology and medicine, 2012, vol. 1, issue 3, pp. 10–14. (in Ukrainian)
  12. Shatorna V., Haretz V., Savenkova O., Kolosova I. The influence of heavy metals and nanometals to the condition of reproductive function in experiment. Tavrycheskyy medyko-byolohycheskyy vestnyk, 2013, vol. 16, issue 1–1, pp. 246–250. (in Ukrainian)
  13. Silva-Zacarin E. C. M., Chauzat M. P., Zeggane S., Drajnudel P., Schurr F., Faucon J. P., Malaspina O., Engler J. A. Protocol for optimization of histological, histochemical and immunohistochemical analyses of larval tissues: application in histopathology of honey bee. Current Microscopy Contributions to Advances in Science and Technology, 2012, pp. 696–703.
  14. Vasiliadi G. K. The development of queen bees and factors affecting their quality. Rossagropromizdat, 1991, 77 p. (in Russian)
  15. Voynalovich M. V., Brovarsky V. D. Morphological changes in ovaries of the honeybees queens. Livestock in Ukraine, 2008, vol. 10, pp. 35–40. (in Ukrainian)

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