Bìol. Tvarin, 2019, volume 21, issue 2, pp. 7–10


J. Aladrović1, B. Beer Ljubić2, R. Laškaj3, L. Vranković1, M. Lojkić4

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1University of Zagreb, Faculty of Veterinary Medicine, Department of Physiology and Radiobiology,
Heinzelova ul., 55, Zagreb, 10000, Croatia
2University of Zagreb, Faculty of Veterinary Medicine, Internal Diseases Clinic,
Heinzelova ul., 55, Zagreb, 10000, Croatia
3University Hospital for Infectious Diseases “Dr Fran Mihaljevic”,
Mirogojska cesta 8, Zagreb, 10000, Croatia
4University of Zagreb, Faculty of Veterinary Medicine, Reproduction and Obstetrics Clinic,
Heinzelova ul., 55, Zagreb, 10000, Croatia

Oxidative stress is important for promoting the oocyte maturation and ovulation within the follicle. The aim of the study was to examine activities of the superoxide dismutase (SOD), glutathione peroxidase (GPx) and biological antioxidant potential (BAP) involved in protection against free oxygen radicals and concentration of reactive oxygen metabolites (ROMs) in bovine follicular fluid.

Bovine ovaries were obtained from a slaughterhouse. The stage of estrous cycle (follicular or luteal) was identified. Follicular fluids (FF) were collected by puncture from three categories of follicles: small (≤5 mm), medium (6–10 mm) or large (>10 mm) from ovaries in both follicular and luteal stage of estrous cycle.

The results indicate a significantly higher activity of SOD in bovine FF from follicles in luteal stage of estrous cycle than in follicular stage (P=0.037). In FF of follicles in both stages of estrous cycle, SOD activity and BAP were significantly higher in FF of small and medium sized follicles than in large ones (P=0.000 both). The ROMs significantly declined from small to large follicles in FF collected during both, luteal and follicular phase of estrous cycle (P=0.005 both). Activity of GPx showed no significant differences regarding neither estrous cycle nor size of follicles.

Results indicate similar antioxidative properties of bovine follicles during both luteal/follicular stage of estrous cycle with higher SOD activity in FF of follicles in luteal stage of estrous cycles which undergo atresia.


  1. Basini G., Bussolati S., Santini S. E., Grasselli F. Reactive oxygen species and anti-oxidant defences in swine follicular fluids. Reproduction, Fertility and Development, 2008, vol. 20, issue 2, pp. 269–274. https://doi.org/10.1071/RD07147
  2. Combelles C. M. H., Holick E. A., Paolella L. J., Walker D. C., Wu Q. Profiling of SOD isoenzymes in compartments of the developing bovine antral follicle. Reproduction, 2010, vol. 139, issue 5, pp. 871–881. https://doi.org/10.1530/REP-09-0390
  3. El-Shahat K. H., Kandil M. Antioxidant capacity of follicular fluid in relation to follicular size and stage of estrous cycle in buffaloes. Theriogenology, 2012, vol. 77, issue 8, pp. 1513–1518. https://doi.org/10.1016/j.theriogenology.2011.11.018
  4. Hozyen H. F., Hodallah H. A., Essawy G. E. S., Shalaby S. I. A. Seasonal changes in some oxidant and antioxidant parameters during folliculogenesis in Egyptian buffalo. Animal Reproduction Science, 2014, vol. 151, issue 3–4, pp. 131–136. https://doi.org/10.1016/j.anireprosci.2014.10.005
  5. Gupta S., Choi A., Yu H. Y., Czerniak S. M., Holick E. A., Paolella L. J., Agarwal A., Combelles C. M. H. Fluctuations in total antioxidant capacity, catalase activity, and hydrogen peroxide levels of follicular fluid during bovine folliculogenesis. Reproduction, Fertility and Development, 2011, vol. 23, issue 5, pp. 673–680. https://doi.org/10.1071/RD10270
  6. Ireland J. J, Murphee R. L., Coulson P. B. Accuracy of predicting stages of bovine estrous cycle by gross appearance of the corpus luteum. Journal of Dairy Science, 1980, vol. 63, issue 1, pp. 155–160. https://doi.org/10.3168/jds.S0022-0302(80)82901-8
  7. LaPolt P. S., Hong L. S. Inhibitory effects of superoxide dismutase and cyclic guanosine 3′,5′- monophosphate on estrogen production in cultured rat granulosa cells. Endocrinology, 1995, vol. 136, issue 12, pp. 5533–5539. https://doi.org/10.1210/endo.136.12.7588305
  8. Miyazaki T., Sueoka K., Dharmarajan A. M., Atlas S. J., Bulkley G. B., Wallach E. E. Effect of inhibition of oxygen free radical on ovulation and progesterone production by the in-vitro perfused rabbit ovary. Journal of Reprodution and Fertility, 1991, vol. 91, issue 1, pp. 207–212. https://doi.org/10.1530/jrf.0.0910207
  9. Revelli A., Piane L. D., Casano S., Molinari E., Massobrio M., Rinaudo P. Follicular fluid content and oocyte quality: from single biochemical markers to metabolomics. Reproductive Biology and Endocrinology, 2009, vol. 7, p. 40. https://doi.org/10.1186/1477-7827-7-40
  10. Roy S. K. Regulation of Ovarian Follicular Development: A Review of Microscopic Studies. Microscopy Research and Technique, 1994, vol. 27, issue 2, pp. 83–96. https://doi.org/10.1002/jemt.1070270203
  11. Sugino N., Takiguchi S., Kashida S., Takayama H., Yamagata Y., Nakamura Y., Kato H. Suppression of intracellular superoxide dismutase activity by antisense oligonucleotides causes inhibition of progesterone production by rat luteal cells. Biology of Reproduction, 1999, vol. 61, issue 4, pp. 1133–1138. https://doi.org/10.1095/biolreprod61.4.1133
  12. Tatemoto H., Muto N., Sunagawa I., Shinjo A., Nakada T. Protection of porcine oocytes against cell damage caused by oxidative stress during in vitro maturation: role of superoxide dismutase activity in porcine follicular fluid. Biology of Reproduction, 2004, vol. 71, issue 4, pp. 1150–1157. https://doi.org/10.1095/biolreprod.104.029264
  13. Whitaker B. D., Knight J. W. Mechanisms of oxidative stress in porcine oocytes and the role of anti-oxidant. Reproduction, Fertility and Development, 2008, vol. 20, issue 6, pp. 694–702. https://doi.org/10.1071/RD08037

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