Bìol. Tvarin, 2018, volume 20, issue 2, pp. 77–81


R. R. Suleimanova
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National University of Life and Environmental Sciences of Ukraine,
15 Heroiv Oborony str., Kyiv 03041, Ukraine

The article presents data on changes in the activity of enzymes: alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ-glutamyltransferase (GGT) in the serum of sterlet of different age groups. As the material for studying the activity of enzymes a serum of one-year-old (weight 0,3–0,4 kg), three-year-old (weight 0.50.6 kg) and nine-year-old (weight 56 kg) sterlet taken in spring period in the fishery “Osetr” (Ukrayinka village Obukhiv district, Kyiv region) was used. Due to the dependence of the enzymes activity in the fish organism on the season and the oxygen regime of the reservoir, in order to minimize the influence of seasonal factors, the selection of samples of tissues was carried out in early May.

The conducted studies have shown that the activity of enzymes with age varies considerably. The study of different age groups of sterlet has shown a significant increase of the ALT activity in serum with age. The ALT activity in three- and nine-year-old sterlet is higher by 1.5 % and by 50.2 %, respectively, than in the two-year-old one. Similar changes were observed in the study of AST activity. The highest activity of the enzyme was observed in fish that had reached the mature age, where it was 42.6 % more than in the two-year-old fish. Since the AST activation is the main link of the malate-aspartate shuttle, there is obviously an increase in its functioning by stimulating the physiological functions of the organism. Bypassing the aspartate aminotransferase of the Krebs cycle causes the active oxidation of not citrate, but succinate, which in turn leads to faster formation of a greater number of ATP molecules than when oxidizing other intermediates of the tricarboxylic acid cycle.

During the analysis of the obtained data, significant changes in the activity of GGT of different age sterlet serum were revealed. In particular, in blood serum of the sexually mature, three- and two-year-old sterlet, the greatest, slightly lower and the lowest activity of GGT was 0.13 μcat/l, 0.06 μcat/l, and 0.05 μcat/l, respectively.

Consequently, the growth of activity of such transferases as ALT, AST, and GGT may be evidence of restructuring the amino acid and protein exchange for the probable energy and plastic adaptation associated with the changes (which are characteristic for the individual development of sterlet) and effect of the environmental and/or alimentary factors.


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