Bìol. Tvarin, 2018, volume 20, issue 1, pp. 123–129

TIME-DEPENDENT CYTOTOXICITY OF CADMIUM CHLORIDE ON CELLS IN VITRO

O. V. Shtapenko, I. I. Gevkan, Yu. I. Slyvchuk

Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

The dynamics of changes of proliferative and metabolic processes in culture of cow fetus embryonic fibroblast cells at different time of influence of cadmium chloride was evaluated. The dependence of cell growth at time of cultivation with cadmium chloride has been established. The proliferative growth of cells was restored after 3 and 6 h of cultivation cells with cadmium chloride, while the prolonger cultivation to 48 and 72 h caused cytotoxic effect. The decrease of cells viability and proliferation after adding the cadmium chloride to the culture medium was accompanied with the metabolism inhibition in cell culture. At the beginning of the stressful action, the total protein content in conditional medium was similar in control and experimental group, whereas prolonged cultivation cells with cadmium chloride for 24, 48 and 72 h led to significant decrease in total protein compared to the control. The glucose content was significantly higher (P<0.001) in conditional medium of the experimental group that indicating about the insignificant level of glucose consumption through a lower level of biosynthetic processes in treatment cells. The decrease the level of exchange processes by the influence of cadmium chloride is confirmed by the dynamics of changes in the concentration of Сalcium and Рhosphorus. Thus, in experimental group the Рhosphorus content in the conditional medium was significantly higher (P<0.001) at 48 h and 72 h of cultivation compared to the control.
The result of our studies indicate that the decline cells viability and proliferation after adding the cadmium chloride to the culture of cow fetus embryonic fibroblast cells cultivation was observed during 2472 h of cultivation however, a more pronounced effect was detected in the long-term effect of the compound.

Keywords: CELL CULTURE, CADMIUM CHLORIDE, PROLIFERATIVE ACTIVITY, CYTOTOXICITY

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