Bìol. Tvarin, 2018, volume 20, issue 1, pp. 123–129


O. V. Shtapenko, I. I. Gevkan, Yu. I. Slyvchuk

Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

The dynamics of changes of proliferative and metabolic processes in culture of cow fetus embryonic fibroblast cells at different time of influence of cadmium chloride was evaluated. The dependence of cell growth at time of cultivation with cadmium chloride has been established. The proliferative growth of cells was restored after 3 and 6 h of cultivation cells with cadmium chloride, while the prolonger cultivation to 48 and 72 h caused cytotoxic effect. The decrease of cells viability and proliferation after adding the cadmium chloride to the culture medium was accompanied with the metabolism inhibition in cell culture. At the beginning of the stressful action, the total protein content in conditional medium was similar in control and experimental group, whereas prolonged cultivation cells with cadmium chloride for 24, 48 and 72 h led to significant decrease in total protein compared to the control. The glucose content was significantly higher (P<0.001) in conditional medium of the experimental group that indicating about the insignificant level of glucose consumption through a lower level of biosynthetic processes in treatment cells. The decrease the level of exchange processes by the influence of cadmium chloride is confirmed by the dynamics of changes in the concentration of Сalcium and Рhosphorus. Thus, in experimental group the Рhosphorus content in the conditional medium was significantly higher (P<0.001) at 48 h and 72 h of cultivation compared to the control.
The result of our studies indicate that the decline cells viability and proliferation after adding the cadmium chloride to the culture of cow fetus embryonic fibroblast cells cultivation was observed during 2472 h of cultivation however, a more pronounced effect was detected in the long-term effect of the compound.


  1. Agbalyan E. V., Shynkaruk E. V. Characterization of the genotoxic and citotoxic effects of small doses of cadmium. International journal of Applied and Fundamental Research, 2015, 6–3, pp. 427–431. (in Russian)
  2. Antonyak H. L., Babych N. O., Biletska L. P., Panas N. E. Cadmium in human and animal organisms. III. Effect on reproductive system. Studia Biologica, 2011, vol. 5, no. 2, pp. 141–152. (in Ukrainian)
  3. Arustamian O. M., Tkachyshyn V. S., Aleksiichuk O. Yu. Influence of cadmium compounds on the human body. Journal of Emergency Medicine, 2016, no. 7, pp. 109–114. (in Ukrainian) https://doi.org/10.22141/2224-0586.7.78.2016.86103
  4. Aziz R., Rafiq M. T., Yang Jie, Liu Di, Lu L., He Z., Daud M. K., Li T., Yang X. Impact assessment of cadmium toxicity and its bioavailability in human cell lines (Caco-2 and HL-7702). BioMed Research International, 2014, p. 8. Article ID 839538. https://doi.org/10.1155/2014/839538
  5. Belyaeva E. A. Cd2+-promoted mitochondrial permeability transition: a comparison with other heavy metals. Acta Biochimica Polonica, 2004, vol. 51, no. 2, pp. 545–551.
  6. Bezruchko N. V., Rubtsov G. K., Grigorieva O. M. Metallothionein: relationship with oxidative modification of proteins and lipids, monitoring methods. Bulletin of Tomsk state university, 2015, vol. 11, no. 164, pp. 161–168. (in Russian)
  7. Dmytrukha N. N. On the problem of immunotoxicity lead and cadmium (literature review). Modern problems of toxicology, 2009, 1, pp. 4–9.
  8. Gordienko V. V. Features of cadmium accumulation in rats of different ages in case of long-term exposition to the salt of metal in low intensity doses. Clinical & Experimental Pathology, 2015, T. XIV, vol. 1, no. 51, pp. 40–43. (in Ukrainian)
  9. Grintsova N. B., Romaniuk A. M. Functional state of pituitary-ovary system of mature female rats following long-term influence of heavy metals salts and non-hormonal injection. ScienceRise: Biological Science, 2017, vol. 3, no. 6, pp. 4–7. (in Ukrainian)
  10. Gzhegotsky M. R., Sukhodolska N. V. Influence of cooper, zinc, cadmium and lead on arising threat of miscarriage in women. Reproductive health. Eastern Europe, 2014, no. 1, pp. 11–17. (in Russian)
  11. Kaminskaya I. A. Adaptive response in human cells with different ability to repair DNA damage Autoref. of PhD thesis in biol. science. Moscow, 1999, 21 p. (in Russian)
  12. Kippler M., Hoque A. M., Raqib R. Accumulation of cadmium in human placenta interacts with the transport of micronutrients to the fetus. Toxicol. Let. J., 2010, vol. 192, pp. 162–168. https://doi.org/10.1016/j.toxlet.2009.10.018
  13. Khyzhnyak S. V. Cell functioning under cadmium intoxication. Modern problems of toxicology, 2009, no. 1, pp. 54–58.
  14. Kolosova I. I. Effect of lead acetate, salts of heavy metals on reproduction. Bulletin of problems in biology and medicine, 2013, vol. 3, no. 6, pp. 4–7. (in Ukrainian)
  15. Krishna A. K., Mohan K. R. Risk assessment of heavy metals and their source distribution in waters of a contaminated industrial site. Environmen. Sci. Pollut. Res. Int., 2013. Available at: http://www.ncbi.nlm.nih.gov/pubmed/24277434.
  16. Madich A., Sheremeta V., Hevkan I., Shtapenko O., Fedorova S., Slyvchuk Yu. Cell culture and its possible use in embryonic biotechnology. A manual for basic technique. Kyiv, ArtEkom, 2012. 144 p. (in Ukrainian)
  17. Marchenko M. L., Bezdenezhnykh N. A., Kudriavets Y. I. Comparative characteristics of the effect of heavy metal compounds on human cells cultivated in vitro. Ukrainian Journal of Occupational health problems, 2008, vol. 3, no. 15, pp. 27–34. Available at: http://opb.org.ua/id/eprint/2479.
  18. Moulis J. M. Cellular mechanisms of cadmium toxicity related to the homeostasis of essential metals. J. M. Moulis Biometals., 2010, vol. 23, no. 5, pp. 877–896. https://doi.org/10.1007/s10534-010-9336-y
  19. Mykhashula G., Sukhodolska N. Maternal and umbilical cord blood levels of lead, cadmium, copper and zinc. Proceeding of the 10th Bialystok International Medical Congress for Young Scientists, Bialystok, 2015, p. 388.
  20. Podolyanska V. V. A complex estimation of children’s health conditions that live on the territory, which is polluted by fluoride and salt of heavy metals. Autoref. of PhD thesis in medical science. Kharkiv, 2001, 16 p. (in Ukrainian)
  21. Pollack A. Z., Ranasinghe S., Sjaarda L. A., Mumford S. L. Cadmium and reproductive health in women: a systematic review of the epidemiologic evidence. Curr. Environ. Helth. Rep., 2014, vol. 1, no. 2, pp. 172–184. https://doi.org/10.1007/s40572-014-0013-0
  22. Rudenko I. V. The role of macro- and microelements in development of congenital malformations. Achievements of Biology and Medicine, 2009, vol. 1, no. 13, pp. 94–98. (in Ukrainian)
  23. Sakamoto M., Yasutake A., Domingo J. L. Relationships between trace element concentrations in chorionic tissue of placenta and umbilical cordtissue: potential use as indicators for prenatal exposure. Environ. Int. J., 2013, vol. 60, pp. 106–111. https://doi.org/10.1016/j.envint.2013.08.007
  24. Shafran L. M., Pykhteev D. M., Bolshoy D. V. Metallothionein as a biomarker in experiment and clinic. Problems of Biological, Medical and Pharmaceutical Chemistry, 2011, no. 9, pp. 60–64. (in Russian)
  25. Sukhodolska N. V. Content of zinc, copper lead and cadmium in system mother-placenta-fetus. Experimental and Clinical Physiology and Biochemistry, 2015, no. 2, pp. 69–77. (in Ukrainian)
  26. Ventskivsky B. M., Osadchuk S. V. Heavy metal content in biological substrates of “Mother-Placenta-Fetus” with the syndrome of fetal growth retardation. J. Medication of Ukraine, 2010, vol. 3, no. 12, pp. 38–41. (in Ukrainian)
  27. Vladimirov Yu. A. Biological membranes and non-programmed cell death. Soros Educational Journal, 2000, no 9, pp. 11–16. (in Russian)
  28. Wan L., Zhang H. Cadmium toxicity: effects on cytoskeleton, vesicular trafficking and cell wall construction. Plant Signal Behav., 2012, vol. 7, no. 3, pp. 345–348. https://doi.org/10.4161/psb.18992
  29. Wang C., Youle R. J. The role of mitochondria in apoptosis. Annual Review of Genetics, 2009, vol.43, pp.95–118. https://doi.org/10.1146/annurev-genet-102108-134850
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