Bìol. Tvarin, 2016, Volume 18, Issue 3, pp. 9–16

http://dx.doi.org/10.15407/animbiol18.03.009

EVALUATION OF ANTIBACTERIAL PROPERTIES OF HETEROCYCLIC COMPOUNDS OF QUINOLONE CLASS AGAINST PASTEURELLA MULTOCIDA

MMBabkina1, LGPalchykovska2, OVVasylchenko2, OMDeriabin3, AATarasov4

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1Institute of Animal Biology NAAS,
38
V. Stus str., Lviv79034, Ukraine

2Institute of Molecular Biology and Genetics National Academy of Sciences of Ukraine,
150 Akademika Zabolotnoho str., Kyiv 03680, Ukraine

3State Scientific Control Institute of Biotechnology and States of Microorganisms,
30 Donetska str., Kyiv 03151, Ukraine

4Institute of Veterinary Medicine NAAS,
30 Donetska str., Kyiv03151, Ukraine

The article describes the results of the study of antibacterial activity and determination of minimum inhibitory concentrations of quinolone class by microdilution method against Pasteurella multocida. The antimicrobial activity of the quinolone class of substances was investigated by disk diffusion method. The existence of antibacterial activity of quinolones to museum test culture of Pasteurella multocida and the presence of the susceptibility in Pasteurella multocida to investigated compounds have been established.

The derivatives of quinolone class which belong to synthetic antibiotic substances of 4-quinolone derivative and contain the piperazin circle in their structure were used. Eleven the most perspective substances with different positions and radical group compositions were choosen that probably strengthened their antimicrobial action toward tested microorganism Pasteurella multocida.

As result of investigations it was identified the minimum inhibitory concentration and growth inhibition zone of substances of chinolone class. The minimum inhibitory concentration was detected at range from 0,41±0,00011 mg/сm3 tо 0,00041±0,000001 mg/cm3 and growth zone diameter was at range from 8,5±0,43 mm to 27,8±0,3 mm.

ODI-39 (9-fluoro-3-methyl-10-(4-methylpiperazin-1-yl)-7-oxo-2,3-dihydro-7H-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylic acid) was the most active compound among the tested substances of quinolone class with a minimum inhibitory concentration 0,00041 mg/cm3 and the diameter of growth inhibition zone 27,8±0,3 mm. The compounds ODI-20, ODI-26, ODI-28, ODI-29, ODI-34, ODI-36, ODI-37, ODI-38 and ODI-43 which differ by nature and position of the radicals demonstrated low level of antibacterial activity with a minimum concentration 0,41 mg/cm3. The diameters of the zones of growth inhibition by these compounds varied from 8,5±0,43 mm to 12,8±0,6 mm. The compounds ODI-16, ODI-17, ODI-18, ODI-19, ODI-21, ODI-22, ODI-23, ODI-25, ODI-27, ODI-30, ODI-31, ODI-32, ODI-33, ODI-35, ODI-40, ODI-41, ODI-42 did not demonstrate any activity against Pasteurella multocida.

Keywords: MICROORGANISM, PASTEURELLA MULTOCIDA, MINIMUM INHIBITORY CONCENTRATION, ANTIBACTERIAL PROPERTIES, QUINOLONES

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