Bìol. Tvarin, 2016, Volume 18, Issue 2, pp. 18–24



V. YuHudyma, I. V. Vudmaska


Institute of Animal Biology NAAS,
38 Stus, str., Lviv 79034, Ukraine

The aim of the study was to determine the metabolic and productive influences of feeding laying hens limestone with different particle size at a constant amount of calcium in the diet. The trial was performed on 30 Hisex brown laying hens of age from 20 to 68 weeks. Hens were divided into 3 groups by 10 hens in each group. All these groups received complete feed differing only with the size of the limestone particles. Hens of 1st group were kept on a diet containing limestone measuring 0–1 mm, 2nd group — 1–2 mm, 3rd group — 2–3 mm. Blood samples were taken from axillary vein on the 20th, 44th and 68th week of life.

Increasing the particle size of limestone was accompanied by an increase of calcium concentration in blood serum, which for an average period of experiment in the 2nd group was 8.58 % higher and in the 3rd group — 10.38% higher than that in the 1st group of hens (P<0.01). Such changes were observed throughout the experiment. In particular, the concentrations of total calcium in the blood serum of hens of 2nd and 3rd groups at the 20th week of age were 10.21 and 7.60 %, at the 44th week — 7.19 and 12.58 %, at the 68th week — 8.58 and 10.38 % higher compared to hens of the 1st group (P<0.05–0.01). The concentration of ionized calcium in the serum increased to a greater extent than the concentration of total calcium. Limestone particle size did not affect the concentration of phosphorus.

With age, the ratio of calcium to phosphorus in the hens’ serum increased which was associated with an increase in the amount of calcium. In the serum of 2nd group hens magnesium concentration rose by 15 %, whereas with increasing particle to 3 mm magnesium content in serum was the same as in hens of 1st group.The blood plasma of 2nd and 3rd groups of hens revealed less alkaline phosphatase activity but this effect was observed only in the beginning of the experiment.


1. Podobed L. I. The value and the physiological role of calcium in the diet of poultry. Modern poultry, 2013, no. 3, pp. 11–12. (in Russian)
2. Podobed L. I. Norms of calcium-phosphorus nutrition of modern poultry hybrids and crosses. Effective poultry, 2006, no. 5, pp. 31–38. (in Russian)
3. Podobed L. I. Guide calcium-phosphorus nutrition of farm animals and birds: a monograph. Odessa, Printing house, 2005, 408 p. (in Russian)
4. Cufadar Y. Effect of alternative calcium sources on performance and eggshell quality in laying hens. JMAS, 2014, vol. 2, no. 1, pp. 10–15.
5. Ekmay R. D., Coon C. N. The effect of limestone particle size on the performance of three broiler breeder purelines. Int. J. Poult. Sci., 2010, vol. 9, no. 11, pp. 1042–1048. https://doi.org/10.3923/ijps.2010.1038.1042
6. Guo X. Y., Kim I. H. Impacts of limestone multi-particle size on production performance, egg shell quality, and egg quality in laying hens. J. Anim. Sci., 2012, vol. 25, no. 6, pp. 839–844. https://doi.org/10.5713/ajas.2011.11468
7. Koreleski J., Swiatkiewicz S. Calcium from limestone meal and grit in laying hen diets-effect on performance, eggshell and bone quality. J. of Animal and Feed Sci., 2004, vol. 72, pp. 1510–1514. https://doi.org/10.22358/jafs/69555/2004
8. Lichovnikova M. The effect of dietary calcium source, concentration and particle size on calcium retention, eggshell quality and overall calcium requirement in laying hens. Br. Poult Sci., 2007, vol. 48, no. 1, pp. 71–75. https://doi.org/10.1080/00071660601148203
9. Pavlovski Z., Lukic M., Spasojevic I. Improving eggshell quality by replacement of pulverised limestone by granular limestone in the hen diet. Acta Vet. Hung., 2003, vol. 53, no. 1, pp. 35–40. https://doi.org/10.2298/AVB0301035P
10. Pizzolante C. C., Kakimoto S. K. Limestone and oyster shell for brown layers in their second egg production cycle. Brazilian J. Poult. Sci., 2011, vol. 13, no. 2, pp. 103–111. https://doi.org/10.1590/S1516-635X2011000200004
11. Roland D. A., Bryant M. Optional shell quality possible without oyster shell. Feedstuffs., 1999, vol. 71, no. 1, pp. 18–19.
12. Safaa H. M., Serrano M. P., Valencia D. G., Frikha M., Jiménez-Moreno E., Mateos G. G. Productive performance and egg quality of brown egg-laying hens in the late phase of production as influenced by level and source calcium in the diet. Poultry Sci., 2008, vol. 87, pp. 2043–2051. https://doi.org/10.3382/ps.2008-00110
13. Saunders-Blades J. L., MacIsaac J. L., Korver D. R., Anderson D. M. The effect of calcium source and particle size on the production performance and bone quality of laying hens. Poultry Science, 2009, vol. 88, pp. 338–353. https://doi.org/10.3382/ps.2008-00278
14. Skrivan M., Bubancova I., Podsednicek M. Influence of limestone particle size on performance and egg quality in laying hens aged 24–36 weeks and 56–68 weeks. Inst. Anim. Sci., 2010, vol. 158, no. 1–2, pp. 110–114. https://doi.org/10.1016/j.anifeedsci.2010.03.018

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