EFFECTS OF ENTEROSORBENTSON THE ACTIVITY OF NO-SYNTHASE IN THE CELLS OF RATS IN THE CONDITIONS OF AFLATOXIN B1 INTAKE
Ch. M. Golovchak1, I. V. Panchuk1, H. L. Antonyak1, 2,O. Ye. Vozna3
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1Institute of Animal Biology, NAAS,
Stusa St., 38, Lviv, 79034, Ukraine
2Lviv Ivan Franko National University,
Lviv, Saksahanskoho St., 1, 79005, Ukraine
3Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj,
Pekarska st., 50, Lviv, 79010, Ukraine
Aflatoxin B1 (AFB1) is one of the most harmful natural toxins that often contaminate crops and animal feed. Intake of AFB1 by animals causes violations of various links of metabolism in blood cells and other tissues and organs. However, the effect of aflatoxin B1 on the system of synthesis of nitric oxide (NO) — an endogenous factor that is involved in the mechanisms of cellular functions regulation is not studied sufficiently. The synthesis of NO from L-arginine occurs by different isoforms of NO-synthase (NOS) in the cells of animals and humans. The aim of the study was to determine the influence of AFB1 on the total NO-synthase activity in red blood cells, kidney cells and mucosa of the small intestine of rats and to investigate the effectiveness of sorbents «Vitakorm-REO» and «Vitakorm-Acidus» in the correction of aflatoxin-related changes in NO synthesis.
The study was conducted on white male rats weighing 250 g, which were divided into 6 groups. The first group was used as control. Rats in group 2 were administered intragastrically by aflatoxin B1 (15mg/kg body weight daily) for 14 days. Animals of the 3rd and 4th groups were injected with AFB1 simultaneously with «Vitakorm-REO» and «Vitakorm-Acidus» respectively. The rats of 5th and 6th groups were given only sorbents with feed.
It was found that the development of experimental aflatoxicosis in animals was accompanied by the changes in the total cellular activity of NO-synthase. On the 7th day of AFB1 intake the activity of NOS in red blood cells of rats increased by 70% and on the 14th day — by 30%. NOS activity in kidney cells increased on the 7th and 14th day of experiment, respectively, by four and two times in comparison to control group. The total activity of NO-synthase increased in the cells of small intestine mucosa of rats to a lesser extent than in the cells of the kidney and erythrocytes. It is shown that the use sorbents «Vitakorm-REO» and «Vitakorm-Acidus» normalizes activity of NO-synthase in the studied cells, and «Vitakorm-REO» is more effective factor for prevention of aflatoxin-related disorders, than «Vitakorm-Acidus».
Keywords: aflatoxin B1, NO-synthase, nitrite, sorbents, rats
