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PROLIFERATIVE GROWTH AND BIOCHEMICAL CHANGES CONDITIONED MEDIUM AS AN INDICATOR OF FUNCTIONALLY MODIFIED COATINGS OF RABBITS OVIDUCTS CELL CULTURE

S. Fedorova¹, O. Shtapenko¹, I Gevkan¹, I. Matiukha¹, M. Zolobko², O. Ogar², Y. Stetsyshyn ², Yu. Slyvchuk¹

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The article describes the use of modified surfaces culture dish as an alternative to conventional plastics with the intensification of proliferative growth and functional activity of the rabbits oviduct cell culture. Accordingly to the tasks of research conditions were reproduced functionality of the tissue cells in vitro. It has been produced a model, which has not only the cells themselves, but all the signaling system which regulates the division, differentiation and functioning of cells in a tissue in vivo. The cell culture was prepared by conventional cold trypsinization, which was tested in laboratories of reproductive biotechnology and breeding animals. Cells were cultured in an incubator at 37 °C, 5 % CO2 and maximal humidity. The modification of the glass surface was carried out by treating the glass plates 3-aminopropyl(triethoxy) silane (APTES), whereby to immobilize them on the primary amino group. On a surface of «clear glass» nanospheres adsorbed bovine serum albumin (BSA) for the creation of biocompatible surfaces and biosensor .

Comparisons were made of the influence of the two species such surfaces — biogel based protein and albumin caused by organic synthesis. By calculating the index of proliferation of the MTT-test, and biochemical studies of conditioned cell culture medium during 72 hours of culture demonstrated that culturing on Biogel natural origin promotes higher viability and metabolic activity of the culture in terms of the activity of lactate dehydrogenase and glucose compared to the use of nano albumin. The results indicate increased vitality and intensity of biosynthetic processes in the cell culture.

Keywords: RABBIT OVIDUCT CELL CULTURE, MODIFIED SURFACE, 3D-CULTURE, GLUCOSE, LACTATE DEHYDROGENASE

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