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Bìol. Tvarin, 2015, volume 17, issue 3, pp. 43–51

PEROXIDATION PROCESSES IN THE BLOOD, LIVER AND SKELETAL MUSCLES OF RABBITS WITH ACUTE L-ARGININE-INDUCED PANCREATITIS AND ITS CORRECTION

O. O. Hopanenko, Y. F. Rivis

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Institute of Agriculture of Carpathian Region National Academy of Agricultural Sciences
M. Hrushevskyi St., 5, Obroshyno, Lviv region., 81115, Ukraine

Aim of this study was to investigate the processes of peroxidation in the blood, liver and skeletal muscles of rabbits with acute L-Arginine-induced pancreatitis and its correction by feeding linseen oil.

The experiment was made in five groups of male rabbits (five animals in each group). The rabbits of the control and I, II, III and IV experimental groups received standard granulated feed during one month. However, during this period rabbits of the I and III experimental groups received daily feed with linseed oil, rabbits of the IV experimental group — feed with sunflower oil in the dose 1 ml/kg of body weight. Also 5 days before the end of the experiment rabbits of the control and experimental groups were made a single intraperitoneal injection of saline sodium chloride in dose 2 ml/kg of body weight and rabbits of the II, III and IV research groups were made an injection of L-arginine in a dose of 4 g/kg of body weight in the same amount of saline. As the material for the research samples of the blood, pancreas, liver and skeletal muscles were used.

It was found that after the intraperitoneal injection of L-arginine the number of necrotic acinar epithelial cells of the head and tail of the pancreas increases and the activity of lipase and α-amylase increases in the blood plasma of rabbits. Feeding up with linseed oil normalizes the number of necrotic acinar epithelial cells of the head and tail of the pancreas and activity of lipase and α-amylase in the blood plasma of rabbits; however feeding up with sunflower oil dramatically increases these indexes. For acute L-Arginine-induced pancreatitis superoxide dismutase and glutathione peroxidase greatly increases, but catalase decreases in the red blood cells, liver and skeletal muscles of the rabbits. The concentration of conjugated dienes, lipid hydroperoxides and malondialdehyde greatly increases in the blood plasma, liver and skeletal muscles of the rabbits with acute L-Arginine-induced pancreatitis. Feeding with linseed oil normalizes while feeding with sunflower oil enhances the vectors activity of superoxide dismutase, glutathione peroxidase and catalase in erythrocytes, liver and skeletal muscles and increases the amount of primary and secondary products of lipid peroxidation in the blood plasma, liver and skeletal muscles of the rabbits for acute L-Arginine-induced pancreatitis.

Keywords: BIOCHEMISTRY, RABBITS, PANCREATITIS, CORRECTION, BLOOD, LIVER, SKELETAL MUSCLE, PANCREAS STATE, PEROXIDATION PROCESSES

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