Bìol. Tvarin, 2017, volume 19, issue 3, pp. 99–106

DYNAMICS OF BIOCHEMICAL INDICES IN BLOOD DURING EARLY PREGNANCY IN RABBITS UNDER THE INFLUENCE OF DIFFERENT FORMS OF STABILIZED GONADOTROPIC PREPARATIONS

Y. I. Slyvchuk1, V. Y. Syrvatka1, I. I. Hevkan1, O. V. Shtapenko1, I. O. Matiukha1, G. O. Milovanova2, O. Y. Slyvchuk1

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1Institute of Animal Biology, NAAS,
38 V. Stusa str., Lviv 79034, Ukraine, This email address is being protected from spambots. You need JavaScript enabled to view it.

2Kyiv National University named after T. Shevchenko,
60 Volodymyrska str., Kyiv 01033, Ukraine

Methodical approaches to creation of new forms of drugs based on stabilized gonadotropins and its activity during prolonged storage were been studied. The influence of injections of stabilized forms of gonadotropin preparations in liposomal forms on the reproductive function of female rabbits during the early stage of pregnancy was determined. The object of the study was human chorionic hormone (hCG) obtained from the urine of pregnant women. The activity of gonadotropin in new forms of drugs stabilized by lysine and sucrose was determined by immuno-chemiluminescent method based on difference between total and free hCG. The effect of stabilizing gonadotropin on reproductive function of female rabbits was determined by the level of estradiol, progesterone and some hematological and biochemical blood parameters in dynamics within two weeks of pregnancy.

It was established that the dispersion leads to loss of 10–12 % of the gonadotropin’s activity however, the duration of dispersion did not influence in its activity. Our results showed that with addition of stabilizers and liposomal form of drug has provided the preservation activity of gonadotropin to 90 % for long-term storage at the temperature of 2–4 °С and at 18–20 °C if taken into account the loss of about 10–12 % after dispersion. The activity of lactate dehydrogenase and concentration of progesterone and estradiol in serum were increased after injections of stabilized preparation during the artificial insemination that indicates its effective action.

KeywordsCHORIONIC GONADOTROPIN, STABILIZATION, RABBIT, PREGNANCY, HAEMATOLOGICAL AND BIOCHEMICAL PARAMETERS

  1. Cole L. A. New discoveries on the biology and detection of human chorionic. Reprod. Biol. Endocrinol., 2009, 7, 8 p. Available at: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2649930.
  2. Frimel G. Immunological methods. Moscow, Medicine, 1987, 215 p. (in Russian)
  3. Larson G. H., Lewis P. E., Dailey R. A. Follicle stimulation hormone pattern and luteal function in cows receiving bovine follicular fluid during three stages of the estrus cycle. Journal of Animal Science, 1987, vol. 64, no. 5, pp. 1491–1497. https://doi.org/10.2527/jas1987.6451491x
  4. Lempiäinen A., Hotakainen K., Alfthan H., Stenman U. Loss of human chorionic gonadotropin in urine during storage at –20 °C. Clinica Chimica Acta, 2012, vol. 413, pp. 232–236. https://doi.org/10.1016/j.cca.2011.09.038
  5. Mc Chesney R., Wilcox A., O’Connor J. Intact HCG, free HCG beta subunit and HCG beta core fragment: longitudinal patterns in urine during early pregnancy. Hum Reprod., 2005, vol. 20, pp. 928–935. https://doi.org/10.1093/humrep/deh702
  6. Pierce J. G., Parsons T. F., Glycoprotein hormones: structure and function. Annu. Rev. Biochem., 1981, 50, pp. 465–495. https://doi.org/10.1146/annurev.bi.50.070181.002341
  7. Slyvchuk Yu. I., Hevkan I. I., Matyukha I. O., Syrvatka V. Ya. Stabilizing the gonadotropin activity with the use of organic compounds. Journal of Microbiology, Biotechnology and Food Sciences, Nitra, Slovakia, 2014, vol. 3, pp. 160–163.
  8. Slyvchuk Yu. I. The use of carbohydrates to stabilize the activity of gonadotropins. Materials of Intern. Scientific-prak. Confer. “Actual problems of scientific and practical veterinary medicine” dedicated to the Vet. Mr., Prof. G. S. Sivkova (March 28–29, 2013). Collection of scientific works of the All-Russian Scientific Research Institute of Veterinary Entomology and Arachnology, Tyumen. Russia, 2013, no. 52, pp. 186–192.
  9. Smolyaninov B. V., Krotkikh M. O. Biotechnology of farm animals. A tutorial. Odessa, 2008, 199 p. (in Russian)

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