Bìol. Tvarin, 2016, Volume 18, Issue 2, pp. 51–58



I M. Kurbatova, M. O. Zakharenko

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National University of Life and Environmental Sciences of Ukraine,
11 Geroiv Oborony str., Kyiv 03041, Ukraine

The effect of anabolic steroids such as nandrolone and deworming albendazole on carp fish depends on their concentrations in the water and is associated with a change in a number of morphological, physiological and biochemical mechanisms in the process of adaptation to the effects of xenobiotics water as it was shown by studies of total and fractional composition of blood plasma protein. The model experiments carried out on the two-year carps to investigate the influence of anthropogenic xenobiotics on the fractional composition of blood plasma proteins revealed that the non-durable exposition (72 hours) and low nanrolone concentration (0.1 mg/dm3 for the first research group and 0.5 mg/dm3 for the second research group) in water in aquariums caused changes in protein content of molecular weight 25, 35–50, 100–140 kDai with constant rates in other fractions. With increase of nandrolone concentration in aquarium water to 1.0 mg/dm3 content of proteins with molecular weight of more than 450 kDa increased by 91 %, 340 kDa— 78 %, 260 kDa — 101 %, 70 kD — 149 %, 50 kDa — 111 %, 25–50 kDa — 35–62 % compared tothe control. We can make the conclusion that anabolic steroids at low concentrations in the water do not affect and at high concentrations stimulate the protein synthesis processes in tissues of carp yearlings.

Non-long stay (12 hours) of carps in the aquarium with a concentration of deworming albendazole 0.2 mg/l in water changed only some of the fractions of blood plasma protein in fish. Increase of concentrations of albendazole in water to 0.5 mg/L and particularly to 1.0 mg/l affected the protein spectrum of blood plasma in fish reducing the content of the most proteins with low and high molecular fractions.

The investigated xenobiotics nandrolone and albendazole at the indicated concentrations in the water and a short exposure did no affect the behavior of fish, the number of respiratory movements and tomographic indicators of their organs.

Status of integument and organoleptic characteristics of the organs in fish of research group under the influence of different concentrations of nandrolone and albendazole did not differ from the similar characteristics in the carps of control group.

The results suggest an important role of fish blood plasma proteins in mechanisms of their adaptation to the effects of water xenobiotics, particularly the anabolic steroid nandrolone and deworming albendazole.


1. Belfroid  A. C., Horst A., Vander Vethaak A. D., Schafer A. J., Rij G. B. J., Wegener J., Cofino W. P. Analysis and occurrence of estrogenic hormones and their glucuronides in surface water and wast Water in the Netherlands. The Science of the Total Environmental, 1999, vol. 225, pp. 101–108. https://doi.org/10.1016/S0048-9697(98)00336-2
2. Tanaka H., Yakou Y., Takahashi A., Komori K., Okayasu Y. Evaluation of environmental estrogens in Japanese. Water Science and Technology, 2001, WEFTEC 2001: Session 51 through Session 60, pp. 632–651 (20).
3. Ching-Hua Huang., David L. Sedlak. Analysis of estrogenic hormones in municipal wastewater effluent and surfaces water using enzymelinked immunosorbent ussey and gas ekromography /tandem mass spectrometry/. Environmental toxicology and chemistry, 2001, vol. 20, no. 1, pp. 133–139. https://doi.org/10.1002/etc.5620200114
4. Gulkowska A., Leung H. W., Yamashita N. Removal of antibiotics from wastewater by sewage treatment facilities in Hong Kong and Sheuzhen, China. Water research, 2008, vol. 42 (1–2), pp. 395–403. https://doi.org/10.1016/j.watres.2007.07.031
5. Bradley P., Barber F., Cray, J. Biodegradation of 17-Estradiol, Estrone and Testosterone in stream Sediments. Environmental Science of Technology, 2009, vol. 43 (13), pp. 1902–1910. https://doi.org/10.1021/es802797j
6. Ivanova A., Zakharenko N. Sanitary-hygienic evaluation of waste water livestock enterprises. Veterinary Biotechnology, 2010, no. 18, p. 77.
7. Ivanova A., Zakharenko N. Sanitary sewage indicators pig enterprises for biological purification methods. Scientific Bulletin of Lviv National University of Veterinary Medicine and Biotechnologies. 2013, no. 3 (57), pp. 335–341.(in Ukrainian)
8. Ivanova A., Novozhytska Y., Zakharenko N, Shevchenko L. Feature sac cumulation Nandrolone and its metabolites in organ sand tissues of rats. Veterinary Medicine of Ukraine, 2011, no. 6. pp. 40–42.(in Ukrainian)
9. Yasojima Makoto, Kobayashi Yoshikazy, Nakacva Norihide, Komori Koya, Suzuki Yutaka, Tanake Hiroaki. Behavior of Human Antibioticsin Wastewater Treatment Plants. Environmental Engineering Research, 2005, vol. 42, pp. 358–368.
10. Takuma Furuichi, Kurunthacha Lamkanna.Occurrence of Estrogenic Compoundsin and Removal by a Swine Farm Waste Treatment Plant. Environmental Science of Technology, 2006, vol. 40, pp. 7896–7902. https://doi.org/10.1021/es0609598
11. Fine D. D., Breidenbach G. P., Price T. L., Hutchins S. R. Quantification of estrogen in groundwater and swine lagoon samples using solid phase extraction, pentafluorobenzyltrimetylsilylduivatizatvions and gas chromatogryfhy-negativ ion chemical ionization tandem mass spertrometry. Chromatogryfhy, 2003, vol. 1017 (1–2), pp. 167–185. https://doi.org/10.1016/j.chroma.2003.08.021
12. Kurbatova I., Tsedyk V. The quality of the water reservoirs of industrial fishing and its impact on the eggs of carp (Cyprinuscarpio L.).Scientific works of Volyn National University, 2012, no. 9, pp. 224–228. (in Ukrainian)
13. Kurbatova I., Tupytska O.,Tsedyk V. Transamination enzyme activity and some chemical parameters of blood carp (Cyprinuscarpio L.) for the actions set xenobiotics. Proceedings of Kalininhrad state technical university, 2014, no. 33, pp. 11–15. (in Russian)
14. Ivanova A. Hygienic assessment juices pig enterprises containing drugs and steroid hormones: dissertation thesis the candidate degree veterinary sciences: specialty 16.00.06 “Animal Hygiene and Veterinary Sanitation”, Kyiv, 2014, 21 p. (in Ukrainian)
15. Tafiychuks R. Analysis of the frequency of micronuclei in erythrocytes carp for the actions of an thelmintics. Scientific Bulletin of Lviv National University of Veterinary Medicine and Biotechnologies, 2008, vol. 10, no. 3 (38), pp. 250–252. (in Ukrainian)
16. Prikhodko Y. Effect of albendazole on protein and carbohydrate metabolism and the state of humoral immunity pigs. VeterinaryMedicine, 2000, vol. 78 (II), pp. 178–181. (in Ukrainian)
17. Baklashova T. Workshop on the ichthyology. Moscow, Agropromizdat, 1990, 223 p. (in Russian)
18. Gornely S. Determination of serum protein by mean of biuret reaction. Journal of Biology Chemistry, 1949, vol. 177, no. 177, pp. 751–755.
19. Laemmly U. K. Cleavage of structural proteins during the assembly of the head of Bacteriophage T4. Nature, 1970, vol. 227, no. 5259, pp. 680–685. https://doi.org/10.1038/227680a0
20. Bondarenko S., Golovin A., Dmytrenko M., Yurchenko A., Babicheva A. Computed registration and analysis of TLC. Chromatography Journal of the Society, 2003, vol. 2, no. 4, pp. 22–30. (in Ukrainian)
21. Kokunin V. A. Statistical data processing with a small number of experiments. Ukrain. biochem. Journal, 1975, vol. 47, no. 6, pp. 776–790. (in Ukrainian)

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