COPPER TOXICOKINETICS IN RATS UNDER PROLONGED ADMINISTRATION OF METAL NANOCOMPOSITES (AG, CU, FE, MN DIOXIDE) WITH FOOD

A. Т. Kutsan, A. L. Orobchenko, M. Ye. Roman’ko

 

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National Scientific Center «Institute of Experimental and Clinical Veterinary Medicine»,

str. Pushkinska, 83, Kharkiv, 61023, Ukraine

 

Experimental model of nanocomposite of metals (NcMe) contained nanoparticles of Argentum (31.5±0.9 nm), Copper (70.0±5.0 nm), Iron (100.0±10.0 nm) and manganese dioxide (50.0±3.0 nm) in aliquots ratio with the final concentration of 100 µg/sm3 for each metal. The concentration of the metals in ionic form in the solution of mixture of salts — AgNO3, CuSO4 • 5H2O, MnSO4 • 5H2O and FeSO4 • 7H2O, respectively was equal to 100 µg/sm3 by metal. The experiment was carried out on mature male rats (n=80) of Vistar line. Observation of animals has been carried out for 90 days. On the 15 th , 30 th , 60 th and 90 th day of feeding the experimental sample NcMe and mixtures of salts of the metals from 5 animals in each group after euthanasia (provided by inhalation anesthetic chloroform) there were sampled organs and tissues, in which there was determined the content of copper by X-ray analysis. By the study of copper toxicokinetics in rats under the conditions of chronic experiment there was determined that metal nanoparticles at introduction in the biotic dose (0.3 mg/kg body weight), have better bioavailability than nano particulate form. For copper nanoparticles in biotic dose functional cumulation is characteristic, whereas for the toxic dose (4.0 mg/kg body weight) — the material cumulation dominates. Excretion of copper, injected to rats in nano- and macro particulate forms in biotic dose stabilized before the end of the experiment, whereas administration of toxic doses resulted in a gradual increase in metal excretion by the kidneys and lungs. Copper accumulation was found in the skin with hair and the testes of experimental rats, which received NcMe.

Keywords:BIOAVAILABILITY, DOSE, NANOCOMPOSITES OF METALS, CUMULATION, COPPER, X-RAY FLUORESCENCE, METAL SALTS, TOXICOKINETICS, RATS

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