dynamics of microstructural changes of white rat liver under the influence of E-selen in complex with Closaverm-A

O. L. Tishyn1, H. I. Kotsiumbas2

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1State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives;11, Donetskst., Lviv, 79019, Ukraine

2Lviv National University of Veterinary Medicine and Biotechnologies after S. Z. Gzhytsky;50,Pekarska st., Lviv, 79010, Ukraine

The aim of the study was to examine the histostructural changes of liver at administration of E-selen in complex with Closaverm-A in therapeutic dose during 14 days. To this end, we used 48 white rats at the age of 2‑3 months with body weight of 170‑185 g. Were used 2 analogous groups with 24 animals in each were formed. The first group was control one. Closaverm‑A in therapeutic dose (0.05 ml/kg) daily during 14 days and E-selen in dose of 0,02 ml/kg at the beginning of experiment and on the 8th day of Closaverm-A administration were administered to animals of the 2nd group. On the 7th and 14th day application and on the 21st and 28th day of recovery period the animals decapitated. The postmortem examination was carried out, liver was weighted, the weight coefficients were determined and collected liver parts and fixed in formalin solution. Dehydration of material and embedding in paraffin were conducted by well‑known method. Thehistocuts were stained with hematoxyline and eosine. At histological test of liver of the 1st control group it was determined that lamellar structure was distinctly expressed. Hepatocytes of  polygonal form with big round nuclei were observed. Duringt histological test of rat liver on the 7thday of administration we observed expressed discomplexation of gulches. In central part of particles radial structure of gulches was expressed, boundary between cells is observed, cytoplasm of hepatocytes is stained, nuclei of round form with chromatine location under karyolemma. On the periphery of particles most of hepatocytes were swollen, their cytoplasm is heterogeneously stained, granular, outlines between cells are degraded, not clear. Hepatocytes with were observed among such cells. On the 14th day in rat liver we observed strengthening of hepatocyte renewal in peripheral area of particles that caused significant increasing of cell content with polyploidy nuclei. New regenerated hepatocytes with intensively stained, basophilic cytoplasm and 2–3 nuclei were observed. It is known that cell polyploidization causes cell division and renewal of functional activity of organ. After stopping of administration on the 21st day renewal of histostructure of hepatocytes and lamellar liver structure were observed. Reparative processes were expressed in whole particle. On the 28th day of renewal period after last administration well-structured lamellar structure of particles was observed. The weight coefficients of liver in experimental animals in comparison with control group on the 7th day of administration of Closaverm-A were decreasing and on the 14th day — were increasing. On the 21st day of renewal, body weight coefficient in the experimental group was decreasing by 10,3 % (p < 0.05), and on the 28th day of renewal we observed insignificant tendency to decreasing.So, according to results of conducted histological tests of white rat liver using Closaverm‑A in therapeutic dose in complex with E-selen, it was determined that on the 7th day of administration we observed the development of dystrophic changes with lamellar structure disorder in peripheral area of particles, on the 14th day of administration — activation of regeneration processes in peripheral area and full renewal of particle structure was observed on the 21st day.

Keywords:Closaverm-A, E-selen, rats, liver, toxicological and pathomorphological tests

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