INFLUENCE OF GERMANIUM CITRATE AND SELENIUM ON LIPID AND HEAVY METALS CONTENT IN MELLIFEROUS BEES’ ORGANISM

R. S. Fedoruk, I. I. Kovalchuk, L. I. Romaniv, M. I. Xrabko

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Institute of Animal Biology NAAS, st. Vasyl Stus, 38, Lviv, 79034, Ukraine

Data is presented on total proteins content and correlation of their separate classes in the melliferous bees’ tissues at feeding them syrup with germanium citrate and germanium with selenium addition. Probable differences between fraction division of lipids and Cu, Ni and Cd content in melliferous bees’ tissues of the experimental groups in comparison to control were established.

Researches were conducted in summer in three groups of melliferous bees three hives each. 1st control group was fed 1000 ml of pure sugar syrup per week; 2nd group in addition to 1000 ml of pure sugar syrup obtained 0.5 mg Germanium in citrate form; 3rd group obtained 0.3 mg of Germanium and 0.2 of Selenium on 1000 ml of sugar syrup per week during 30 days. Total lipids extraction in the melliferous bees’ tissues samples was conducted according to Folch method and their quantity was determined by gravimetric method. Concentration of separate heavy metals (Fe, Сu, Ni, Cr, Zn, Pb і Cd) was determined on atomic absorption spectrophotometer SF-115 PK.

Probably higher total proteins in bees’ tissues of the 2nd and 3rd group (p<0.05) was observed after feeding melliferous bees sugar syrup and Germanium citrate and Selenium addition. Moreover expressed differences in correlation of the separate classes of lipids in experimental groups’ bees’ tissues was established. Including probably higher relative content of phospholipids, non-etherified fatty acids on the background of free and etherified cholesterol (p<0.01) level decrease in the 3rd group bees. The established changes in total lipids and their fractions content in the bees’ organism at feeding them Germanium and Selenium citrates indicate the influence of these compounds on the lipids metabolism in the bees’ tissues.

Feeding Germanium and Selenium citrate with sugar syrup is marked with their antagonistic influence on separate heavy metals(Fe, Cr, Ni, Pb, Cd) level in bees’ tissues. Probably lower content of Fe and Cd was established in experimental group bees’ tissues on the background of higher Cu level.

Obtained results witness about positive changes in the content of separate lipid fraction including processes of metabolic accumulation of energetic and plastic components of the food chain and also mineral elements metabolism in bees’ organism. This proves the necessity of using Germanium and Selenium citrate additions for correction of melliferous bees mineral nutrition.

Keywords: BEES, TISSUES, GERMANIUM AND SELENIUM CITRATES, LIPIDS, HEAVY METALS, GLYCOGEN

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