FORMING OF BIOFILMS OF MICROORGANISMS OBTAINED FROM MILKING EQUIPMENT

M.D. Kukhtyn1, N.V. Krushelnytska2

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1Ternopil Research Station of the Institute of Veterinary Medicine of NAAS,
st. Troleybusna 12, Ternopil, 46027, Ukraine

2State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives,
st. Donetska, 11, Lviv, 79019,
Ukraine

The article presents the test results of bacteria capability, obtained from milking equipment, to form microbial biofilm. The aim of test was to study the influence of efficacy of sanitary processing and detergents on microorganism capability to form biofilms on abiogenic surfaces of milking equipment. The tests conducted on the territory of 3 husbandries where sanitary processing of milking equipment and inventory is conducted by means of different detergents. Selection of washouts from milking equipment, that was previously processed, was conducted before milking of cows.

Studying of microorganism capability to form biofilms and determination of their density were conducted in Petri dishes where daily bacteria test culture in concentration of 105 colony forming units/sm3 with further adding of 5.0 sm3 of culture medium and incubation at 30 °C during 2448 hours. Thecapability to form biofilms was evaluated visually and microscopically, the biofilm density was determined spectrophotometrically in units on the basis of optic density of flushing alcohol solution low density equals to 5 units, medium one is considered to be equal to 0.51 unit, the high density of 1 unit. Under condition of good and satisfactory sanitary processing of milking equipment washout microflora was presented by more than 50 % by gram-negative bacteria of Pseudomonas, Acinetobacter, Alcaligenes, Enterobacter, Eschirichia and Citrobacter and more than 40 % by coccus forms of Staphylococcus, Streptococcus, Enterococcus and Micrococcus and gram-positive bacteria. On insufficiently clean equipment the microflora composition was presented by coccus forms and gram-positive bacteria, their quantity was equal to 70 % of microflora.

The results of conducted tests showed that generic composition of microflora of milking equipment, the density of formed biofilms and contamination of milk by bacteria depended on the quality of sanitary processing and detergents. It was determined that for getting of high quality of milk on milking farms it is necessary to carry out diligent sanitary processing of milking equipment by means of alkaline and acid disinfectants after every milking of cows independently of the technology of milk getting.

Keywords: MILKING EQUIPMENT, MILK, MICROBIAL BIOFILMS, SAFETY

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