Bìol. Tvarin, 2014, volume 16, issue 1, pp. 83–89

THE EFFECT OF THIOTRIAZOLIN IN DOG SEMEN FREEZING EXTENDER ON SPERM PLASMA MEMBRANE AND ACROSOME INTEGRITY AFTER THAWING

A. R. Korbetskyy, M. M. Sharan

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Institute of Animal Biology NAAS,
38 Stus str., Lviv 79034, Ukraine

In this article are given the study results of addition of thiotriazolin to dog semen cryopreservation extender at different concentrations (2, 4, 6, 8, 10 and 12 mg/ml) compared with medium without addition of thiotriazolin. The changes of sperm progressive motility, longevity, plasma membrane and acrosome integrity of spermatozoa at different thiotriazolin concentrations added to the dog semen freezing extender. The ejaculates of nine healthy mature dogs of different breeds were used in the experiment. As a result of the study it was found that thiotriazolin at concentration of 2 and 4 mg/ml did not cause an increase in the progressive motility of spermatozoa after thawing compared to control. Established, that increasing the concentration of thiotriazolin to 6 mg/ml led to a significant increase (p˂0.05) the number of progressive spermatozoa after thawing by 7.6 % compared to control. Further increase in thiotriazolin concentration (8, 10 and 12 mg/ml) in sperm freezing extender caused increased number of spermatozoa with progressive movement after thawing with high probability (p˂0.001) compared to control, but between them we did not observe significant difference, which indicates no positive impact of thiotriazolinon sperm motility after thawing with increased concentrations from 8 to 12 mg/ml. Increased concentration of thiotriazolin between 10 and 12 mg/ml tended to improve the number of sperm with intact plasma membrane by 11.7 and 14.3 % (p˂0.001) respectively. Studying the rate of spermatozoa longevity after thawing frozen in the media with different thiotriazolin concentrations we observed positive effect of thiotriazolin starting from concentration of 4 mg/ml, where the value of this index was by 6.9 % higher (р<0.05) compared to control.

Keywords: DOGS SPERM, CRYOPRESERVATION, PLASMA MEMBRANE INTEGRITY, ACROSOME PRESERVATION, THYOTRIAZOLIN

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