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MOLECULAR-GENETICCHARACTERIZATION OF SHETLAND PONY USING MICROSATELLITE LOCI OF DNA

O. V. Melnyk, V. G. Spyrydonov, V. V. Dzitsiuk, S. A. Osadchyj

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National University of Life and Environmental Sciences of Ukraine

Ukraine, Kyiv, Heroyiv Oborony st., 15, 03041

The aim of the present work was to conduct a genetic analysis of 12 goals of Shetland pony with known pedigree by microsatellite DNA loci for individual identification, parentage testing and population-genetic characterization of the selected livestock. In a study genomic DNA was extracted from whole blood. PCR was performed by 7 microsatellite loci of DNA (HTG04, HTG06, HMS06, HMS02, AHT04, ASB17, ASB23), recommended by the International Society of Animal Genetics (ISAG). Amplification products were denatured formamide and separated by electrophoresis on a genetic analyzer. A totalnumber of 37 alleles were detected. The number of detected alleles per locus ranged from 4 (HTG06) to 7 (ASB23)with the mean number 5.29. The mean value of observed heterozygosity (0.619) was lower than expected (0.723), indicating the prevalence in the studied group of animals homozygous genotypes. Despite the fact that all analyzed loci were polymorphic (PIC>0.550), the most polymorphic locus was ASB23 (0, 812). In turn, the locus HMS02 had the lowest value of PIC (0.589). Combined exclusion probability was 99.04%. Conducted genetic analysis has shown the efficiency of selected microsatellite loci of DNA for individual identification, parentage testing and population-genetic characterization of Shetland pony. In future available is genetic analysis of more number of pony of this breed using additional microsatellite DNA loci.

Keywords: SHETLAND PONY, MICROSATELLITES, GENETIC ANALYSIS, POLYMORPHISM, POLYMERASE CHAIN REACTION, HETEROZYGOSITY, ALLELE FREQUENCY

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