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THE USE OF IMMUNOGENETICS MARKERS FOR EXPOSURE OF COWS RESISTANT OR SENSIBLE TO MASTITIS

T. Suprovich1, V. V.Vlizlo2

1Podolsky State Agrarian Technical University, Kamenetz-Podolsk, 32316

T. Shevchenko str. 13, kokas2008@ukr.net

2Institute of animal biology NAAS, Lviv — 79034, 79034, V. Stusa str. 38

In the article the possibility of application of molecular-genetic methods is brought with the use of main complex of histocompatability in diagnostics of cow mastitis.

The problem of conventional breeding cattle is the duration estimates cows. With the advent of genetic markers appeared to accelerate the pace of selection. It is known that susceptibility to mastitis cows genetically conditioned trait. It directs research efforts to find genetic markers associated with resistance or susceptibility to mastitis cows. It becomes possible to predict the manifestation of the disease in the early stages of postnatal ontogenesis.

To improve the accuracy of prediction should combine several tagging signs of this disease, as disease resistance factor (which in most cases mastitis) depends on various mechanisms of pathogenesis.

With the discovery of major histocompatibility complex the prospect to identify molecular genetic markers immediately after birth and cows on the basis set for the populations of previous studies of markers to predict phenotypic expression of the disease in the future.

Research is conducted on the cows of the Ukrainian red-pied breed. For the exposure of «informing» antigens of class1 and alleles of gene of DRB3 BoLA-system was created base from the group of resistant and receptive to cows udder diseases. Authentication of antigens of class1 of BoLA-system was conducted by a standard two-stage microcitotoxic test after Kissmeyer-Nielsen in modification for a cattle. Spectrum by the alleles of gene of BoLA-DRB3 studied by means of PCR-analysis. By the complex analysis of "informing" antigens, educed in population and on results statusmetric and biometric analysis was identified BoLA-antigens related to mastitis, which can be used as markers qualifying receptivity (W6 and A16) and resistance (A17 and А19) to the disease. Biometric analysis of frequency spectrum of alleles of gene of BoLA-DRB3 showed that with receptivity to cows udder diseases of red-pied breed is associated with alleles of gene of BoLA-DRB3*07 and *08. Close connection with resistance is shown by alleles *22 and *24.

Keywords: COWS, MASTITIS, MAJOR HISTOCOMPATIBILITY COMPLEX, MOLECULAR-GENETIC MARKERS, ANTIGENS, ALLELES, STATUSMETRIYA

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