Bìol. Tvarin, 2013, volume 15, issue 4, pp. 134–140


S. Fedorova1, O. Shtapenko1, I Gevkan1, I. Matiukha1, M. Zolobko2, O. Ogar2, Y. Stetsyshyn 2, Yu. Slyvchuk1

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1 Institute of animal biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine, This email address is being protected from spambots. You need JavaScript enabled to view it.
2National University “Lviv Polytechnics”, VIII teach. building,
2 St. George sq., Lviv 79013, Ukraine

The article describes the use of modified surfaces culture dish as an alternative to conventional plastics with the intensification of proliferative growth and functional activity of the rabbits oviduct cell culture. Accordingly to the tasks of research conditions were reproduced functionality of the tissue cells in vitro. It has been produced a model, which has not only the cells themselves, but all the signaling system which regulates the division, differentiation and functioning of cells in a tissue in vivo. The cell culture was prepared by conventional cold trypsinization, which was tested in laboratories of reproductive biotechnology and breeding animals. Cells were cultured in an incubator at 37 °C, 5 % CO2 and maximal humidity. The modification of the glass surface was carried out by treating the glass plates 3-aminopropyl(triethoxy) silane (APTES), whereby to immobilize them on the primary amino group. On a surface of «clear glass» nanospheres adsorbed bovine serum albumin (BSA) for the creation of biocompatible surfaces and biosensor .

Comparisons were made of the influence of the two species such surfaces — biogel based protein and albumin caused by organic synthesis. By calculating the index of proliferation of the MTT-test, and biochemical studies of conditioned cell culture medium during 72 hours of culture demonstrated that culturing on Biogel natural origin promotes higher viability and metabolic activity of the culture in terms of the activity of lactate dehydrogenase and glucose compared to the use of nano albumin. The results indicate increased vitality and intensity of biosynthetic processes in the cell culture.


  1. Brynda E., Houska M., Jirouskova M. Albumin and heparin multilayer coatings for blood-contacting medical devices. Journal of Biomedical Material Research, 2000, 51, pp. 249–257.<249::AID-JBM14>3.0.CO;2-X
  2. Brynda E., Houska M. Ordered multilayer assemblies: Albumin/heparin for biocompatible coatings and monoclonal antibodies for optical immunosensors. In: Lvov Y., Mohwald H., eds. Protein Architecture: Interfacing Molecular Assemblies and Immobilization Technology. New York, Marcel Dekker, 2000, pp. 251–286
  3. Ladam G., Schaaf P., Decher G. Protein adsorption onto auto-assembled polyelectrolyte films. Biomolecular Engineering, 2002, 19, pp. 273–280.
  4. Miksa D., Irish E. R., Chen D. Dextran Functionalized Surfaces via Reductive Amination: Morphology, Wetting, and Adhesion. Biomacromolecules, 2006, 7, pp. 557–564.
  5. Rabe M., Verdes D., Seeger S. Surface-induced spreading phenomenon of protein clusters. Soft Matter, 2009, 5, pp. 1039–1047.
  6. Sapsford K. E., Ligler F. S. Real-time analysis of protein adsorption to a variety of thin films. Biosensors and Bioelectronics, 2004, 19, pp. 1045–1055.
  7. Yu S. Y., Hu J. H., Pan X. Y. Stable and pH-sensitive nanogels prepared by selfassembly of chitosan and ovalbumin. Langmuir, 2006, vol. 22, pp. 2754–2759.
  8. Stetsyshyn Y. Study of the adsorption of albumin on the surface-modified glass by elipsometry. Scientific journal “Visnyk of L’viv University”, Biological Series, 2010, no. 54, pp. 51–58. (in Russian)
  9. Shtapenko O. The proliferation of cows oviduct cell culture and some biochemical parameters of the medium under the action of nickel chloride. Scientific and technical bulletin of Institute of Animal Biology and State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, 2009, 10 (1–2), pp. 342–346. (in Russian)
  10. Benny Abraham Kaipparettu, Isere Kuiatse1, Bonita Tak-Yee Chan1, Meju Benny Kaipparettu. Novel egg white-based 3-D cell culture system. BioTechniques, 2008, vol. 45, pp. 165–171.
  11. Pampaloni, F., Reynaud E. G., Stelzer E. H. The third dimension bridges the gap between cell culture and live tissue. Nature Reviews Molecular Cell Biology, 2008, vol. 8, pp. 839–845.
  12. Alon R., Hershkoviz E., Bayer A. Streptavidin blocks immune reactions mediated by fibronectin-VLA-5 recognition through an Arg-Gly-Asp mimicking site. European Journal of Immunology, 1993, vol. 23, pp. 893–898.
  13. Madich A. V. Cell culture and their possible use in embryonic biotechnology. A Textbook. Kyiv, ArtEkonom, 2012, 144 p.

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