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PCR-DIAGNOSTICATION OF SEVERE COMBINED IMMUNODEFICIENCY

DISEASE (SCID) IN HORSES

Y. F. Kurylenko1, S. O. Kostenko1, O. V. Dubin1, M. I. Zhezher2

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1National Universityof Life and Environmental Sciences of Ukraine,

Heroiv Oborony str., 15; Kyiv, 03041, Ukraine

2NF PJSC «Rayz-Maksimko», avenue Peremogi, 121 v, 03115, Kyiv

The severe combined immunodeficiency (SCID) is a hereditary disease of the Arabian and cross-breed horses, characterized by a complete loss of the immune response due to a mutation in the gene for DNA-dependent protein kinase C. Protein kinase C is one of the most important enzymes involved in the reconstruction of the gene segment in the synthesis and T-cell receptor. The foals are born healthy but die of any infections since ceased functioning colostral antibodies, and its own immune system does not work properly. SCID is manifested only in the homozygous state and is characterized by 100 % mortality, while heterozygous animals do not have any phenotypic manifestations of the disease and are carriers. Therefore, the aim of this work was the genetic monitoring of Arabian breed horses of Yagilnitskiy stud farm for the presence of SCID mutation.

The material for the study was the hair follicles of 16 Arabian horses. Studies carried out using PCR using specific primers with which the desired gene fragment is amplified size 136 or 131 bp from healthy individuals and mutant respectively. Electrophoretic separation of the amplification products was performed in 8% native polyacrylamide gel electrophoresis (PAGE) followed by analysis of the lengths of the amplified fragments. Investigations was conducted with using PCR followed by analysis of the lengths of the amplified fragments. The result of monitoring Arabian horse breed was the presence of mutations in DNA-dependent protein kinase C (DNA-PKc) of test animals showed no carriers SCID. It is planned to continue research on other populations of the Arabian breed of horses in Ukraine.

Keywords: ARABIAN HORSE BREED, IMMUNODEFICIENCY, MUTATION, DNA-PKсs, POLYMERASE CHAIN REACTION

 


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