the relative proportion of T-and B-lymphocytes and their functional activity of female-nursery carps depending on the level of vitamin A in the ration

І. M. Popyk, O. I. Vischur

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Institute of Animal Biology NAAS, st. Stusa 38, Lviv, 79034, Ukraine

One of the most actual scientific and practical issues of contemporary pond fish farming is to increase the resistance of fish to diseases and negative anthropogenic environmental factors. In this regard, the development of effective methods of carps feeding, considerable attention is given to the search of the immune status of fish. In the specialized fish farms fish breeding is closely connected with its feeding, which aim is to get maximal number of high quality products in the shortest terms for the least feed cost. It is important to ensure a complete fish ration with vitamins, especially vitamin A.

The purpose of the search ascertain the influence of different levels of vitamin A in the ration of female-nursery carps on the activity of T-and B-cell immune units. The search was conducted on three groups of female-nursery scaly carps, to which during before-spawning period, vitamin A was carried in, in the form of retynilacetate at doses of 2500 IU and 5000 IU. It was determined that additional input into the content of the ration of female-nursery carps vitamin A is finding out the stimulating effect on the number of T-and B-lymphocytes, as well as on their functional activity through the redistribution of lymphocyte receptor system in the direction of increasing their avidity. In particular, the number of T-lymphocytes (total, active, theophylline-resistant and-susceptible) and B-lymphocytes in the blood of female-nursery carps, which in addition to the feed were fed with vitamin A was greater than that of female-nursery carps, who consumed only a standard feed. It was found out a greater number of T-and B-lymphocytes in the blood of female-nursery carps from experimental groups compared to the control.

Keywords: cellular stages of immunity, T-lymphocytes, B-lymphocytes, female-nursery, carp, DOSE, vitamin A, RETYNILATSETAT

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