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Bìol. Tvarin, 2013, volume 15, issue 1, pp. 126–133

EFFECT OF SILVER NANOPARTICLES ON RABBIT’S GAMETES IN VITROAND FERTILIZATION PROCESSES IN VIVO

V. J. Syrvatka, Y. I. Slyvchuk, I. I. Rozgoni, I. I. Gevkan

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Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

The aim of the study was to investigate the influence of silver nanoparticles on rabbit’s gametes in vitro and fertilization processes in vivo.

 For this purpose, we used a commercially available silver nanoparticles (synthesized by electrochemical method) and nanoparticles synthesized in laboratory via chemical reduction. Westudied the effect of different concentrations of silver nanoparticles (0, 0.01, 0.1, 1, and 10 mg/ml) in culture medium on rabbit’s oocytes maturation co-cultured with granulosa cells and on sperm viability and motility during 72 hours of storage at 15 °C. The efficiency of artificial insemination in presence of silver nanoparticles in sperm extender at concentration of 0.1 mg/ml was determined for study of their effect on the fertilization process in vivo.

 It was revealed, that silver nanoparticles at the concentration of 0.01 to 10 µg/ml hadno negative impact on the maturation of oocytes in vitro. However, in concentration 10 µg/ml silver nanoparticles caused a decrease in viabilityof granulosa cells and change in biochemical parameters of conditioned medium. The results showed that silver nanoparticles at the concentration of 0.01 to 1µg/ml did not influencedupon rabbit’s sperm motility and viability. Nevertheless, presence of silver nanoparticles in sperm extender at a concentration of 10 µg/ml led to reduction of sperm motility and viability during 72 hours of storage. The concentration of silver nanoparticles 0.1 µg/ml in a semen extender had no influence on the number of pregnant rabbits and newborn per doe.

Thus, concentration of 1 µg/ml of silver nanoparticles is critical, increases of this concentration are potentially toxic on animal’s reproductive system.

Keywords: SILVER NANOPARTICLES, RABBITS, OOCYTES, SPERMATOZOA, FERTILITY IN VIVO

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