Bìol. Tvarin, 2019, volume 21, issue 3, pp. 87–91


O. Jaremko, R. Pelenio

This email address is being protected from spambots. You need JavaScript enabled to view it.

Lviv National University of Veterinary Medicine and Biotechnologies named after S. Gzhytsky,
50 Pekarska str., Lviv 79010, Ukraine

We examined the content of vitamin B6 in colostrum and milk of cows which was fed to calves and its concentration in blood calves from 1 to 90 days for the exogenous administration of different doses of pyridoxine hydrochloride. For the experiment, 6 groups of calves (control and five experimental), 5 animals in each group, were formed. During the experiment (from 1 to 90 days), the animals of the control and experimental groups were in identical feeding, care and maintenance conditions, and their rations were balanced in accordance with the rules of feeding young animals of the dairy period of cultivation. Calves of the control group were fed the main diet (MD), while in the experimental group, pyridoxine hydrochloride was added to the main diet (MD): Group I 1 mg/kg body weight, II 2, III  3, IV 4 and V group 5 mg/kg of body weight. In the experiment, pyridoxine hydrochloride was used in powder, which was prescribed once a day during morning feeding. The material for the research was fresh-milk colostrum and milk of cows and blood of calves born by them. Samples were taken at 1, 5, 21, 60 and 90 days after calving of cows and, accordingly, at 1, 5, 21, 60 and 90 days of life of calves. It has been found that vitamin B6 content in colostrum and milk of cows given to calves of the studied groups was highest at the 1st day after calving and was 31.4 mg/kg. At day 5, its concentration decreased to 15.6 mg/kg, and at the 21st day 19.6 times. Up to 60 days, a slight decrease in the content of vitamin B6 in milk was detected, and at 90 days after calving of cows its concentration was 0.6 mg/kg of milk, which was found to be lower by 52.3 times, compared to the 1st day. The content of vitamin B6 in the blood of calves in the dairy period of cultivation also turned out to be the highest at the 1st day of their lives, then decreased to 21st day and increased to the 90th day. For additional administration to the diet of pyridoxine hydrochloride, a probable growth (P<0.05), compared with the control group, of the content of vitamin B6 at 5th and 21st day at doses of 2, 3, 4 and 5 mg/kg of body weight and on 60th and 90th day for doses of 3, 4 and 5 mg/kg of body weight.The optimal dose of daily supplement of pyridoxine hydrochloride for correction of vitamin-supply in calves at age 1–21 days is 4 mg/kg of body weight, in calves at age 21–90 days 3 mg/kg of body weight.


  1. Ahmed L., Nazrul Islam S. K., Khan M. N. I., Huque S., Ahsan M. Antioxidant micronutrient profile (vitamin E, C, A, Copper, Zinc, Iron) of colostrum: association with maternal characteristics. Journal of Tropical Pediatrics, 2004, vol. 50, issue 6, pp. 357–358. DOI: 10.1093/tropej/50.6.357.
  2. Halias V., Kolotnytskyi A., Fedets O. The biological role of vitamins in the body of animals. Lviv, 2006, 80 p. (in Ukrainian)
  3. Hnoievyi I. V. Feeding and reproduction of livestock in Ukraine. Institute of Animal Husbandry UAAS, Kharkiv State Animal Veterinary Academy of the Ministry of Agrarian Policy of Ukraine. Kharkiv, Competition, 2006, 400 p. (in Ukrainian)
  4. Hrevtsev A. Vitamin nutrition of calves. Milk & feed. Management, 2007, no. 4, pp. 14–16. (in Russian)
  5. Ibatullin I. I., Panasenko Yu. O., Kononenko V. K. Workshop on feeding farm animals. A textbook for studio. Kyiv, Higher Education, 2003, pp. 178–190. (in Ukrainian)
  6. Kalashnykov A. P., Fysynyn V. F., Shchehlov V. V., Kleimenov N. Y. (eds.). Norms and Diets for Feeding Farm Animals. A reference book. 3rd Moscow, Rosselkhozacademia, 2003, 422 p. (in Russian)
  7. Levchenko V. I. (ed.), Vlizlo V. V., Kondrakhin I. P., Melnychuk D. O., Apukhovska L. I., Halias V. L. (ed.), Holovakha V. I., Sakhniuk V. V., Tomchuk V. A., Hryshchenko V. A., Tsvilikhovskyi M. I. Veterinary Clinical Biochemistry. A reference book. Bila Tserkva, 2002, pp. 192–200. (in Ukrainian)
  8. Lutsenko O. I., Voron N. M. The role of vitamins in human life. Young Scientist, 2017, issue 2 (42), pp. 7–13. Available at: http://molodyvcheny.in.ua/files/journal/2017/2/3.pdf (in Ukrainian)
  9. Magne-B6 Anti-stress: composition, indications, dosage, side effects. Available at: https://www.obozrevatel.com/health/lekarstva/magne-v6-antistress.htm (in Russian)
  10. Mazurkevych A. Y., Karpovskyi V. I. (eds.), Kambur M. D., Trokoz V. O., Stepanchenko L. M. Physiology of Animals. A textbook. 2nd Vinnytsya, The New Book, 2012, 420 p. (in Ukrainian)
  11. Melikian S. M., Solohub L. I. Age features of metabolism in microorganisms of cattle rumen. Experimental and Clinical Physiology and Biochemistry, 2006, issue 4, pp. 33–36. (in Ukrainian)
  12. Newsholme E., Leech A. Functional Biochemistry in Health and Disease. Wiley-Blackwell, 2010, 544 p.
  13. Parkhomenko Yu. M., Donchenko H. V. Vitamins in human health. Kyiv, Academyperiodica, 2006, 182 p. (in Ukrainian)
  14. Spyrychev V. B. Theoretical and practical aspects of modern vitamins. Questions of Nutrition, 2005, vol. 74, issue 5, pp. 32–50. (in Russian)
  15. Suslova N. I., Shkvaria M. M. Physiological state of newborn calves and their resistance to diseases. Scientific herald of the Lviv National University of Veterinary Medicine and Biotechnology named after S. Gzhytsky, Lviv, 2010, vol. 3, issue 2 (2), pp. 318–322. (in Ukrainian)
  16. Vazhnycha O. M. Anti-stress action of pyridoxine as a manifestation of its pharmacodynamic activity. Pharmacology and Drug Toxicology, Kyiv, 2007, p. 31. (in Ukrainian)
  17. Vlizlo V. V. (ed.), Fedoruk R. S., Ratych I. B. Laboratory Methods of Research in Biology, Livestock and Veterinary Medicine. A reference book. Lviv, 2012, 764 p. (in Ukrainian)
  18. Zhehunov H. F., Yakymenko T. I., Prykhodchenko V. O., Hladka N. I., Denysova O. M. Vitamin. A methodical manual. Kharkiv, Kharkiv State Animal Veterinary Academy, 2017, 24 p. (in Ukrainian)

Download full text in PDF




WorldCat Logo