Bìol. Tvarin, 2019, volume 21, issue 2, pp. 7–10

ANTIOXIDATIVE SYSTEM OF BOVINE FOLLICLES REGARDING STAGE OF ESTROUS CYCLE AND FOLLICULOGENESIS

J. Aladrović1, B. Beer Ljubić2, R. Laškaj3, L. Vranković1, M. Lojkić4

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1University of Zagreb, Faculty of Veterinary Medicine, Department of Physiology and Radiobiology,
Heinzelova ul., 55, Zagreb, 10000, Croatia
2University of Zagreb, Faculty of Veterinary Medicine, Internal Diseases Clinic,
Heinzelova ul., 55, Zagreb, 10000, Croatia
3University Hospital for Infectious Diseases “Dr Fran Mihaljevic”,
Mirogojska cesta 8, Zagreb, 10000, Croatia
4University of Zagreb, Faculty of Veterinary Medicine, Reproduction and Obstetrics Clinic,
Heinzelova ul., 55, Zagreb, 10000, Croatia

Oxidative stress is important for promoting the oocyte maturation and ovulation within the follicle. The aim of the study was to examine activities of the superoxide dismutase (SOD), glutathione peroxidase (GPx) and biological antioxidant potential (BAP) involved in protection against free oxygen radicals and concentration of reactive oxygen metabolites (ROMs) in bovine follicular fluid.

Bovine ovaries were obtained from a slaughterhouse. The stage of estrous cycle (follicular or luteal) was identified. Follicular fluids (FF) were collected by puncture from three categories of follicles: small (≤5 mm), medium (6–10 mm) or large (>10 mm) from ovaries in both follicular and luteal stage of estrous cycle.

The results indicate a significantly higher activity of SOD in bovine FF from follicles in luteal stage of estrous cycle than in follicular stage (P=0.037). In FF of follicles in both stages of estrous cycle, SOD activity and BAP were significantly higher in FF of small and medium sized follicles than in large ones (P=0.000 both). The ROMs significantly declined from small to large follicles in FF collected during both, luteal and follicular phase of estrous cycle (P=0.005 both). Activity of GPx showed no significant differences regarding neither estrous cycle nor size of follicles.

Results indicate similar antioxidative properties of bovine follicles during both luteal/follicular stage of estrous cycle with higher SOD activity in FF of follicles in luteal stage of estrous cycles which undergo atresia.

Keywords: BOVINE FOLLICULAR FLUID, ANTIOXIDATIVE SYSTEM, LUTEAL STAGE OF ESTROUS CYCLE, FOLLICULAR STAGE OF ESTROUS CYCLE

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