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Bìol. Tvarin, 2018, volume 20, issue 2, pp. 30–42

STRUCTURAL CHANGES IN RATS KIDNEYS UNDER THE INFLUENCE OF HISTAMINE AND SODIUM HYPOCHLORITE

N. P. Harasym, O. I. Bishko-Moskalyuk, A. M. Shumska, D. I. Sanahursky

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Lviv National University named after Ivan Franko,
4 Hrushevskoho str., Lviv 79005, Ukraine, This email address is being protected from spambots. You need JavaScript enabled to view it.

Influence of histamine and sodium hypochlorite (SH), as well as their simultaneous action on structural features of rat kidneys on the 1^st, 7^th, 14^th day of the experiment and after the rehabilitation period (21^st day) is investigated using light microscopy and morphometric analysis.

It has been established that the exogenous administration of histamine rats at a dose of 1 μg/kg results in a decrease in the area of the kidneys corpuscles, vascular glomeruli at the 7^th day, whereas the higher dose of the nutrient amine leads to morphometric indices in both 1^st and 7^th day of the experiment. It has been found that this biogenic amine causes narrowing of the lumen of the proximal and distal convoluted tubules in the cortical kidney. Under these conditions, cells are poorly perceived as a color, optically opaque, indicating the presence of metabolic changes.

SH at a concentration of 5 mg/l causes an increase in the area, as well as a larger and smaller axis of the vascular lobe on the 7^th day of the experiment, while this substance in higher concentrations leads to significant cellular impairment for 1 day of the experiment. SH causes the development of hydropic dystrophy, disturbances in the structure of the membranes, as evidenced by their fuzzy contouring, increased permeability of blood vessels and capillaries. Changes in the size of the vasculature glomerular indicate a violation of the filtration process in the kidneys.

Under the combined effect of SH and histamine there is a decrease in the area of the kidney corpuscle, an increase in the area of the vascular glomerule, damaged cell membranes, distal and proximal tubules, increased vascular permeability, development of hydropsic dystrophy. Probably the decrease in the area of the kidney cells is due to damage to the cells of the proximal and distal tubules, the increase of which leads to compression of the Shumlyansky-Bowman capsule. Under the action of histamine, hydrogen peroxide, ammonia is formed, which leads to a toxic kidney damage. The simultaneous introduction of histamine and SH into the body rats is also likely to produce halogen derivatives that can affect kidney cells. These changes are less pronounced with the simultaneous action of SH and histamine at a dose of 1 μg/kg.

Keywords: HISTAMINE, SODIUM HYPOCHLORITE, KIDNEY, MORPHOMETRY

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