Bìol. Tvarin, 2015, volume 17, issue 3, pp. 124–131

STATE OF RAT BONE TISSUE AT CONDITION OF NITRITE INTOXICATION AND CORRECTION OF VIOLATIONS BY ARTICHOKE EXTRACT

N. S. Khopta

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Ivano-Frankivsk National Medical University,
Galytska str., 2, Ivano-Frankivsk, 76014, Ukraine

In view of strengthening the role of environmental factors in causing bone tissue (BT) pathology, topical study of the effect of nitrite on bone metabolism was presented. Nitrite intoxication (1/10 LD50 sodium nitrite) was modeled on the white male rats (1-st and 2-nd group of animals) for 10 days, and then the animals of group 2 were administered to correct of artichoke extract (EA). Biopsy specimens (blood, thigh bones) were performed in 1-, 14- and 28-days after the administraion of toxicant. In blood plasma markers of bone metabolism were determined, in bone ash — Ca, Mg, Zn, Cu and Cd content, femur mineral density (BMD) — by densitometry. The results were compared with intact animals.

Under the administration of nitrite-ions in rats there was a change in the rate of phosphorus-calcium metabolism: the concentration of Ca2+ and phosphate increased by 82%; Mg decreased by 44,5–55,4 %; alcaline phosphatase activity decreased by 2,6 times, and ACP increased by 1,9 times. This rate of catabolism of collagen caused the concentration of hydroxyproline to increase by 13,5 %, parathyroid hormone level increased by 8,9 % and calcitonin decreased by 6,2 times. Balance index of processes of osteosynthesis and resorption — index ALP/ACP decreased by 3,13 times. Bioelementry composition of BT infected animals changed as follows: reduced content of Ca by 14,4–26,9 %, Zn by 20,6–23,6 %, Cu — by 5,25 times, and Mg increased by 26,0 % to 1-st day, and then reduced to that of intact. Gradual increase of the amounts of heavy metal Cd (1,3–2,1 times) and reduced BMD (20,5–28,3 %) were observed.

On the background of EA using the tendency to normalize the concentration of Ca, Mg, hydroxyproline, PTH, calcitonin and the index ALP/ACP were observed. However, the level of phosphate still remains slightly higher (16,5–20,5 %). Content of osteotropic bioelements by the end of observation were not significantly different from the these paramethes in the rats of control group and Cd reduced by 35.7 %. It was found the significantly increase of BMD.

Keywords: WHITE RATS, BONE TISSUE, SODIUM NITRITE, ARTICHOKE EXTRACT, PHOSPHORUS-CALCIUM METABOLISM, BIOELEMENTS, BONE MINERAL DENSITY

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