Bìol. Tvarin, 2015, volume 17, issue 3, pp. 97–103

THE USE OF THE SPIN PROBE REDUCTION FOR THE EASTIMATION OF THE RESPIRATORY CHAIN ACTIVITY OF THE CARDIOMYOCYTES AND HEPATOCYTES MITOCHONDRIA OF THE RATS UNDER THE ARTIFICIAL HYPOBIOSIS

S. D. Melnytchuk1, S. V. Khyzhnyak1, O. A. Nardid2, Ya. O. Cherkashyna2, V. S. Morozova1, V. Riepina2, V. M. Voitsitsky1

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1National university of life and environmental sciences of Ukraine, Kyiv, 03041 Heroyiv Oborony str., 15, Ukraine

2Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, 61015, Pereyaslavska str., 23, Ukraine

The results of researches of the activity of oxidation-reduction reactions of the respiratory chain of the hepatocytes and cardiomyocytes mitochondria in the rats by the spin probe (TEMPON) electron paramagnetic resonance (EPR) was contented. The artificial hypobiosis state (the influence of hypothermia, hypercapnea and hypoxia) in the animals was created by Bakhmet’ev-Giay-Anjus model. The animals were decapitated in the state of the artificial hypobiosis (rectal temperature of 16 °C) and in 2 and 24 hours after the termination of the influence of the artificial hypobiosis factors (rectal temperature of 37 °C as a control). As a parameter of spin probe reduction the relative changes of mean field component amplitude of probe EPR spectra depending on time were used. The values of the speed constant of the spin probe reduction in the samples of the inner mitochondrial membrane were calculated by the tangent of the angle in the coordinates (lgI, t).

As it was found, the reduction of the spin probe (by the value of the reduction speed constant) in the interaction with the respiratory chain of the hepatocytes under the artificial hypobiosis is similar to the respective control for the investigated temperature range (16-37 °C). However, the reduction of the spin probe in the interaction with mitochondrial respiratory chain of the cardiomyocytes under the artificial hypobiosis increases at all experimental temperatures in comparison to the corresponding control. Perhaps under the artificial hypobiosis the activation of the complex II (succinate-CoQ-oxidoreductase) of the respiratory chain of the cardiomyocyte mitochondria is done leading to the increase of redox activity of its sites which are engaged in a reduction of the probe. The changes are of short duration, because of the investigated parameters are near to the control values in a day after the influence of the factors which cause the artificial hypobiosis state.

Keywords: HYPOBIOSIS, CARDIOMYOCYTES, HEPATOCYTES, MITOCHONDRIA, RESPIRATORY CHAIN, ELECTRON PARAMAGNETIC RESONANCE, SPIN PROBE

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