Bìol. Tvarin, 2017, volume 19, issue 3, pp. 50–54

ANALYSIS OF CYTOKINS STATUS IN BLOOD SERUM OF COWS WITH SUBCLINICAL FORM OF of KETOSIS

D. V. Kibkalo

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Kharkiv state zooveterinary academy, HDZVA,
Mala Danylivka, Derhachivsky district, Kharkiv region, 62341, Ukraine

Levels of pro-inflammatory interleukins IL-1 and IL-6, anti-inflammatory IL-4 in blood serum at subclinical ketosis in cows did not differentiate from levels in clinically healthy animals.

The researches were conducted on cows of red-and-white milk breed with productivity above 6000 l during lactation. The clinical inspection, sampling of urine and blood were conducted in first weeks of lactation. Content of ketone bodies in blood was determined by ketometer Optium Xceed (USA), in urine it was by indicated stripes Ketophan (ERBA Lachema). By the results 2 groups were formed: an experimental one — cows with ketone bodies in urine and level 1.12.1 mmol/l in blood serum, and a control group — in urine ketone bodies were not detected, in blood serum their level was 0.40.7 mmol/l. In blood serum the content of α1-, α2-globulins, levels of IL-1, IL-4, IL-6 were determined. Their researches were conducted on IFA ELISA with using of test-system “Penisula laboratories Inc.(USA).

At subclinical process of ketosis the level of pro-inflammatory interleukins -1 and -6 in blood serum was not varied from level of clinically healthy animals. It testifies the absence of inflammatory processes at subclinical form of ketosis. It is confirmed by normal level α1- and α2-globulins, because just IL-6 lead to increasing of proteins of “acute phase” of inflammatory, which are included in their content. The content of IL-4 did not vary significantly in control and experimental groups of cows. The content of leukocytes in animals of both groups was in normal borders that also confirms the absence of inflammatory process at subclinical form of ketosis in cows.

Keywords: COWS, KETOSIS, SERUM OF BLOOD, CYTOKINS, INTERLEUKINS, KETONE BODIES

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