DIVERSITY AND THE DISTRIBUTION OF ALLELES OF BOLA-DRB3.2 GENE AMONG CATTLE BREEDS

T. Suprovych1, M. Suprovych1, R. Kolinchuk1, N. Makhnochova2

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1Podilsky State Agrarian Technical University,
13 T. Shevchenko str., Kamyanets-Podilskyi 32316, Ukraine

2Institute of Animal Breeding and Genetics named after M. Zubets,
1 Pogrebnyak str., Chubynske, Boryspil district, Kyiv region 08321, Ukraine

The article presents a study of polymorphisms of gene BoLA-DRB3.2 in two native breeds (Ukrainian black-pied dairy and red-pied dairy) in comparison with other world breeds of cattle. We used methods PCR-RFLP and AS-PCR. Detect 54 alleles exon 2 gene BoLA-DRB3 allow these methods. In Ukrainian, black-pied dairy cattle have found 28, and the red-pied dairy — 22 alleles. Frequency spectrum of alleles in both populations is uniform. There are determined alleles at frequency of more than 5%: in Ukrainian black-pied dairy cattle *03, *08, *10, *13, *22, *24 and *28; in Ukrainian red-pied dairy — *01, *03, *07, *11, *16, *22 and *24.Ukrainian breeds have a high enough level of polymorphism. The value of expected heterozygosis for Ukrainian black-pied dairy breed He = 0,939, and for the Ukrainian red-pied dairy — He = 0,921.

Research shows diversity of the allelic spectrum for various world breeds in the main regions where have dairy cattle breeding. There are presence of high levels polymorphism and genetic diversity in investigated breeds, as well as in most breeds of dairy cows of the world. Obviously, DRB3.2 locus is informative molecular genetic marker. It can be used in breeding expected to create herds resistant to several diseases. Necessary to expand such research in the Ukraine in numerical terms and in the range of breeds of cattle, localized in the state.

Keywords: UKRAINIAN BLACK-PIED DAIRY CATTLE, UKRAINIAN RED-PIED DAIRY CATTLE GENE BOLA-DRB3, POLYMORPHISM, ALLELES, POLYMERASE CHAIN REACTION, MOLECULAR GENETIC MARKER

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