Bìol. Tvarin, 2015, volume 17, issue 4, pp. 69–75

STIMULATION OF NOGALAMYCINE PRODUCTION BY COEXPRESSION OF SNORA AND RELA GENES IN STREPTOMYCES NOGALATER LV 65

DOKlymyshyn

klymyshyn.d@gmail.com

Institute of Animal Biology NAAS,
34 Stus str., Lviv 79034, Ukraine,
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Danylo Halytsky Lviv National Medical University,
69 Pekarska str., Lviv 79010, Ukraine,
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Anthracycline antibiotics are an important group of anticancer compounds widely used in tumor chemotherapy. They are used to treat different types of diseases such as leukemias, lymphomas, breast, uterine, ovarian and lung cancers. Investigation of the regulatory mechanisms of antibiotic production in Streptomyces is of great interest, as these data provide a potential platform for generation of industrially important strains to increase the production of their secondary metabolites. Streptomyces nogalaterLv65 is a producer of ananthracycline antibiotic nogalamycine. Derivatives of this antibiotic are used in chemotherapy of tumors. Here we focused on expression of snorA and relA genes in S. nogalater Lv65. A set of plasmids for the expression of snorA and relA genes were generated. Conjugation procedures of the S. nogalater Lv65 and UV33, S. echinatus and S. peucetius wild-type strains with pKCAII and pVWBA1 were carried out from E. coli ET12567 (pUB307). An increasing in the nogalamycin production was observed when pKCAII and pVWBA1 plasmids were introduced into the Lv65 and UV33 strains. We believe that this indicates a crucial role for snorA and relA in nogalamycin biosynthesis under some nutritional conditions. This is in agreement with the general knowledge about aforementioned regulatory genes. Expression of snorA and relA genes in S. echinatus Lv 22 and S. peucetius subsp. caesius producers of aranciamycine and doxorubicin respectively, stimulates antibiotic production in the above strains.

Keywords: Antibioticbiosynthesis, STREPTOMYCESNOGALATER, NOGALAMYCINE, SARPPROTEINS

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