Bìol. Tvarin. 2020; 22(1): 10–14.
https://doi.org/10.15407/animbiol22.01.010
Received 26.12.2019 ▪ Accepted 22.03.2020 ▪ Published online 01.05.2020

Energy supply of laboratory rats tissues at combined action of cadmium chloride and sodium nitrite

LD. Kuras, H. M. Erstenyuk
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Ivano-Frankivsk National Medical University,
2 Halytska str., Ivano-Frankivsk, 76018, Ukraine

The mechanism of action of Cadmium and nitrites is different: Cadmium inhibits the enzyme systems of energy supply by replacing divalent metals; and nitrites enhance the formation of methemoglobin, which disrupts oxygen transport to cells. However, to date, the combined effect of these xenobiotics, in particular on energy metabolism, remains poorly understood. Taking into account the foregoing, the purpose of this study was to find out the state of energy metabolism in the brain and liver of rats under conditions of combined action of cadmium chloride and sodium nitrite. The intoxication was modeled as follows: cadmium chloride was administered intramuscularly. Sodium nitrite was administered with drinking water at a dose of 1/10 of LD50 once a day for 10 days. Animals were divided into two groups: intact and experimental. The material was collected after decapitation under thiopental anesthesia at 1st, 14th, 28th day after the completion of the introduction of toxicants. Indicators of energy metabolism were determined as follows: activity of ATPase, lactate dehydrogenase — enzymatic method; glucose concentration — glucose oxidase method; the level of pyruvic, lactic, adenosine triphosphate (ATP) acids — spectrophotometrically; content of Zn, Cu, Mg — by atomic absorption spectrophotometer. The conducted researches indicate disturbance of energy supply of brain and liver tissues by cadmium-nitrite intoxication, which is confirmed by activation of aerobic oxidation of glucose in the brain, increase of ATP content and activity of ATPase in the investigated organs.

Key words: rats, energy metabolism, cadmium chloride, sodium nitrite, Na+, K+-activation, Mg2+-dependent ATPase, adenosine triphosphate, glucose, lactate dehydrogenase, liver, brain

  1. Al-Rasheed NM, Fadda LM, Attia HA, Ali HM, Al-Rasheed NM. Quercetin inhibitssodium nitrite-induced inflammation and apoptosis in different ratsorgansby suppressing Bax, HIF1-α, TGF-β, Smad-2, and AKT pathways. Biochem Mol Toxicol, 2017; 31(5): 55–60. DOI: 10.1002/jbt.21883.
  2. Bahri S, Kaddour H, Karoui D, Bouraoui S, Amri M, Mokni M. Protective role of vitamin E against cadmium induced oxidative stress into the rat liver. Tunis Med. 2019; 97(1): 100–105.
  3. Chmylenko FO, Derkach TM. Methods of atomic spectroscopy: Atomic-absorption spectral analysis: teaching manual. Dnipro, RVV DNU; 2002: 120 p. (in Ukrainian)
  4. Danilovich GV, Gruzina TG, Ulberg ZR, Kosterin SO. Identification and catalytic properties of Mg2+-dependent ATP-hydrolase of plasmic membrane of Bacillus sp. B4253 capable to gold accumulation. Ukr. Biochem. Jour. 2004; 76(5): 45–51. (in Ukrainian)
  5. Derecha LM. Macro- and trace elements: modern ideas about their functional significance in the warm-blooded organism. Experimental and Clinical Medicine. 2007; 4: 21–27. (in Ukrainian)
  6. Hunchak VM. To the toxicology of nitrates and nitrites in animals. Scientific Bulletin of the S. Z. Gzytsky. 2013; 15(3/1): 62–70. (in Ukrainian)
  7. Levchenko VI, Holovakha VI, Kondrakhin IP. Methods of laboratory clinical diagnostics of animal diseases. Kyiv, Ahrarna osvita. 2010: 437 p. (in Ukrainian)
  8. Maiboroda R. Computer statistics — professional start. Kyiv, Kyivskyi universytet. 2018: 482 p. (in Ukrainian)
  9. Melnychuk DO, Melnychuk SD, Kalachniuk LH, Shevriakov MV, Kalachniuk HI. Biochemistry: a workshop. Kyiv, NULES of Ukraine. 2012: 528 p. (in Ukrainian)
  10. Pohorielov MV, Bumeister VI, Tkach HF, Bonchev SD, Sikora VZ. Macro- and microelements (metabolism, pathology and methods of determination): monograph. Sumy, SumDU Publ., 2010: 147 p. (in Ukrainian)
  11. Salama MF, Abbas A, Darweish MM, El-Hawwary AA, Al-Gayyar MMH. Hepatoprotective effects of cod liver oil against sodium nitritetoxicity inrats. Pharm Biol. 2013; 51(11): 1435–43. DOI: 10.3109/13880209.2013.796564.
  12. Skalnyy AV, Rudakov IA. Bioelements in medicine. Moscow, ONIX 21 Century Publishing House. 2004: 272 p. (in Russian)
  13. Skliarov OY, Fartushok NV, Soika LD, Smachylo IS. Biological chemistry with biochemical methods of research: textbook. Kyiv, Medicine. 2009: 352 p. (in Ukrainian)
  14. Trakhtenberg I.M. Problems of norm in toxicology: modern concepts and methodical approaches, basic parameters and constants. Moscow, Meditsina. 1991: 206 p. (in Russian)
 

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