Bìol. Tvarin, 2019, volume 21, issue 4, pp. 58–64


I. V. Lobachova, V. S. Yakovchuk

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Institute of Animal Husbandry in Steppe Regions “Ascania-Nova” named after M. F. Ivanov’s — National Scientific Selection-Genetic Centre of Sheep Breeding,
1 Soborna str., Ascania-Nova, Chaplynskyy district, Kherson region, 75230, Ukraine

Most breeds of sheep exhibit seasonality of reproduction. To make it possible to inseminate outside the estrous season, ewes are subjected to hormonal stimulation. The basic scheme of stimulation includes the treatment with gonadotropins after preliminary gestagenization. The most suitable form of prolonged saturation of the body with gestagens is the vaginal sponge. However, the effectiveness of such a scheme is not always sufficient. It is suggested that one of the reasons may be irritation of the vaginal walls with the material of sponge, and one of the ways to reduce this is the treatment of animals with substances that affect the different parts of the nervous system. The purpose of the experiment was to compare the effectiveness of the basic scheme of sexual activity stimulation in sheep during anoestrous season with the scheme that was added by the use of a substance with anticholinesterase activity “Proserine”. For this purpose 2 groups of 2-year-old ewes (control and experimental) were formed and kept together. Gestagenization of all animals was initiated on 11 April by the insertion of sponges with gestagen (Cronolone, 30 mg/ sp., “Intervet”), which were removed on 23 April. To stimulate folliculogenesis, on April 22 all animals were injected with 500 IU of the “Sergon” (PMSG, Czech Republic) and 1 ml of 2.5 % oil solution of progesterone. In addition, all animals were twice treated with 2.5 ml of “SuperAmino-C” (South Korea), which is a mixture of essential amino acids and B vitamins, in the days of insertion and withdrawal of sponges. “Proserine” (0.05 % solution) was injected the experimental animals at a dose 1 ml per ewe per injection twice — in the days of insertion and withdrawal of sponges. All animals were artificially inseminated with freshly obtained semen one time per day during three consecutive days, starting at the next day after sponge withdrawal, without the previous heat testing. In the experimental group, 91.6 % of animals lambed, in the control one — 70.6 %. The prolificacy in the experimental group was 1.18±0.19 vs. 1.68±0.34 lambs/ewe in the control. The fecundity at lambing in the experimental group was 108.5 % vs. 118.5 % in the control, at weaning at 2-months old — 83.3 % vs. 74.1 %. The results of the experiment showed that addition to the basic scheme the 2-fold treatment with the antiesterase substance “Proserine” reduces prolificacy, but increases the efficiency of insemination and improves the survival of lambs to weaning that generally contributes to the increased efficiency of hormonal stimulation in sheep. It was made assumption that the positive effect of the neurotropic substance was able to manifest itself due to the simultaneous saturation of the animal organism with a mixture of essential amino acids and B vitamins.


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